Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2895655..2896157 Replicon chromosome
Accession NZ_CP029973
Organism Escherichia coli strain 51008369SK1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DNQ41_RS14865 Protein ID WP_000767829.1
Coordinates 2895655..2895909 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DNQ41_RS14870 Protein ID WP_001259255.1
Coordinates 2895906..2896157 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNQ41_RS14835 2890670..2890897 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DNQ41_RS14840 2890911..2891969 - 1059 WP_087523589.1 transport protein YeeE -
DNQ41_RS14845 2892148..2893506 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DNQ41_RS14850 2893496..2893558 - 63 WP_010723108.1 membrane protein YoeI -
DNQ41_RS14855 2893773..2894702 - 930 WP_000803366.1 LysR family transcriptional regulator -
DNQ41_RS14860 2894748..2895572 - 825 WP_000754737.1 SDR family oxidoreductase -
DNQ41_RS14865 2895655..2895909 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DNQ41_RS14870 2895906..2896157 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DNQ41_RS14875 2896441..2896491 + 51 WP_001364200.1 his operon leader peptide -
DNQ41_RS14885 2896637..2897536 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DNQ41_RS14890 2897542..2898846 + 1305 WP_074514295.1 histidinol dehydrogenase -
DNQ41_RS14895 2898843..2899913 + 1071 WP_000108933.1 histidinol-phosphate transaminase -
DNQ41_RS14900 2899913..2900980 + 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T106775 WP_000767829.1 NZ_CP029973:c2895909-2895655 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T106775 NZ_CP029973:c2895909-2895655 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT106775 WP_001259255.1 NZ_CP029973:c2896157-2895906 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT106775 NZ_CP029973:c2896157-2895906 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References