Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1017628..1018322 Replicon chromosome
Accession NZ_CP029973
Organism Escherichia coli strain 51008369SK1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag DNQ41_RS05085 Protein ID WP_001263489.1
Coordinates 1017924..1018322 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag DNQ41_RS05080 Protein ID WP_000554758.1
Coordinates 1017628..1017921 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNQ41_RS05060 1013260..1013757 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
DNQ41_RS05065 1013981..1015693 - 1713 Protein_913 flagellar biosynthesis protein FlhA -
DNQ41_RS05070 1015665..1016450 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
DNQ41_RS05075 1016521..1017576 + 1056 WP_001226164.1 DNA polymerase IV -
DNQ41_RS05080 1017628..1017921 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
DNQ41_RS05085 1017924..1018322 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
DNQ41_RS05090 1018332..1018784 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
DNQ41_RS05095 1019102..1019308 + 207 Protein_919 RtcB family protein -
DNQ41_RS05100 1019304..1019825 + 522 Protein_920 peptide chain release factor H -
DNQ41_RS05105 1019882..1021339 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
DNQ41_RS05110 1021600..1022058 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 1022654..1022734 + 81 NuclAT_12 - -
- 1022654..1022734 + 81 NuclAT_12 - -
- 1022654..1022734 + 81 NuclAT_12 - -
- 1022654..1022734 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T106762 WP_001263489.1 NZ_CP029973:1017924-1018322 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T106762 NZ_CP029973:1017924-1018322 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT106762 WP_000554758.1 NZ_CP029973:1017628-1017921 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT106762 NZ_CP029973:1017628-1017921 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References