Detailed information of TA system TA106701

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 217341..217767
• Replicon: plasmid pMCR1-PA
• Accession: NZ_CP029748
• Organism: Escherichia coli strain 2016C-3878

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BEN53_RS26660
• Protein ID: WP_001312861.1
• Coordinates: 217341..217499 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	217543..217767 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN53_RS26620	212713..213402	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
BEN53_RS26625	213589..213972	-	384	WP_001151524.1	relaxosome protein TraM	-
BEN53_RS26630	214293..214895	+	603	WP_072156291.1	transglycosylase SLT domain-containing protein	-
BEN53_RS26635	215192..216013	-	822	WP_001234469.1	DUF945 domain-containing protein	-
BEN53_RS26640	216132..216419	-	288	WP_000107535.1	hypothetical protein	-
BEN53_RS27390	216444..216650	-	207	WP_000547971.1	hypothetical protein	-
BEN53_RS26660	217341..217499	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	217543..217767	-	225	NuclAT_0	-	Antitoxin
-	217543..217767	-	225	NuclAT_0	-	Antitoxin
-	217543..217767	-	225	NuclAT_0	-	Antitoxin
-	217543..217767	-	225	NuclAT_0	-	Antitoxin
BEN53_RS27255	217579..217767	+	189	WP_001299721.1	hypothetical protein	-
BEN53_RS26665	217779..218498	-	720	WP_000116352.1	plasmid SOS inhibition protein A	-
BEN53_RS26670	218495..218929	-	435	WP_064770609.1	conjugation system SOS inhibitor PsiB	-
BEN53_RS26675	218984..219181	-	198	Protein_237	hypothetical protein	-
BEN53_RS26680	219209..219442	-	234	WP_000005987.1	DUF905 family protein	-
BEN53_RS26685	219510..220049	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
BEN53_RS27395	220075..220281	-	207	WP_000275856.1	hypothetical protein	-
BEN53_RS26710	221420..222391	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / mcr-1.1 / mph(A) / fosA3 / blaCTX-M-14 / aac(3)-IVa / aph(4)-Ia / floR / sitABCD / blaCTX-M-55	iutA / iucD / iucC / iucB / iucA	1..276880	276880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T106701 WP_001312861.1 NZ_CP029748:c217499-217341 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T106701 NZ_CP029748:c217499-217341 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT106701 NZ_CP029748:c217767-217543 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin