Detailed information of TA system TA106696

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 179010..179264
• Replicon: plasmid pMCR1-PA
• Accession: NZ_CP029748
• Organism: Escherichia coli strain 2016C-3878

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BEN53_RS26425
• Protein ID: WP_001351576.1
• Coordinates: 179010..179216 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	179203..179264 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN53_RS26385	174562..174963	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
BEN53_RS27250	174896..175153	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
BEN53_RS26390	175246..175899	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
BEN53_RS27380	175997..176137	-	141	WP_001333237.1	hypothetical protein	-
BEN53_RS26400	176838..177695	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
BEN53_RS26405	177688..178170	-	483	WP_001273588.1	hypothetical protein	-
BEN53_RS26410	178163..178237	-	75	WP_001442103.1	RepA leader peptide Tap	-
BEN53_RS26420	178469..178726	-	258	WP_000083845.1	replication regulatory protein RepA	-
BEN53_RS26425	179010..179216	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	179203..179264	+	62	NuclAT_1	-	Antitoxin
-	179203..179264	+	62	NuclAT_1	-	Antitoxin
-	179203..179264	+	62	NuclAT_1	-	Antitoxin
-	179203..179264	+	62	NuclAT_1	-	Antitoxin
BEN53_RS27385	179520..179594	-	75	Protein_189	endonuclease	-
BEN53_RS26435	179840..180052	-	213	WP_001299730.1	ANR family transcriptional regulator	-
BEN53_RS26440	180188..180748	-	561	WP_000139315.1	fertility inhibition protein FinO	-
BEN53_RS26445	180851..181711	-	861	WP_000704513.1	alpha/beta hydrolase	-
BEN53_RS26450	181770..182516	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / mcr-1.1 / mph(A) / fosA3 / blaCTX-M-14 / aac(3)-IVa / aph(4)-Ia / floR / sitABCD / blaCTX-M-55	iutA / iucD / iucC / iucB / iucA	1..276880	276880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T106696 WP_001351576.1 NZ_CP029748:c179216-179010 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T106696 NZ_CP029748:c179216-179010 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT106696 NZ_CP029748:179203-179264 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin