Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2109936..2110498 Replicon chromosome
Accession NZ_CP029747
Organism Escherichia coli strain 2016C-3878

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag BEN53_RS10870 Protein ID WP_000605676.1
Coordinates 2109936..2110214 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag BEN53_RS10875 Protein ID WP_000781370.1
Coordinates 2110214..2110498 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEN53_RS10840 2105522..2105953 - 432 WP_000152305.1 peroxiredoxin OsmC -
BEN53_RS10850 2106298..2106513 + 216 WP_000495766.1 biofilm-dependent modulation protein -
BEN53_RS10855 2106615..2106752 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
BEN53_RS10860 2106909..2108606 + 1698 WP_000433464.1 malate dehydrogenase -
BEN53_RS10865 2108740..2109750 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
BEN53_RS10870 2109936..2110214 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BEN53_RS10875 2110214..2110498 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
BEN53_RS10880 2110549..2111202 - 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
BEN53_RS10885 2111195..2112079 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -
BEN53_RS10890 2112092..2115139 - 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T106684 WP_000605676.1 NZ_CP029747:2109936-2110214 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T106684 NZ_CP029747:2109936-2110214 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCAGATATCGATATGCCCGGTTACCGATTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTCGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT106684 WP_000781370.1 NZ_CP029747:2110214-2110498 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT106684 NZ_CP029747:2110214-2110498 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References