Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 914383..915062 Replicon chromosome
Accession NZ_CP029747
Organism Escherichia coli strain 2016C-3878

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag BEN53_RS04645 Protein ID WP_000057541.1
Coordinates 914383..914685 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag BEN53_RS04650 Protein ID WP_000806442.1
Coordinates 914721..915062 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEN53_RS04615 909646..910866 - 1221 WP_001251634.1 fosmidomycin MFS transporter -
BEN53_RS04625 911084..912736 + 1653 WP_110826415.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
BEN53_RS04630 912773..913252 - 480 WP_000186626.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
BEN53_RS04640 913456..914250 - 795 WP_000365157.1 TraB/GumN family protein -
BEN53_RS04645 914383..914685 + 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BEN53_RS04650 914721..915062 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
BEN53_RS04655 915120..917624 - 2505 WP_061092784.1 copper-exporting P-type ATPase CopA -
BEN53_RS04660 917887..918819 + 933 WP_000883039.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T106674 WP_000057541.1 NZ_CP029747:914383-914685 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T106674 NZ_CP029747:914383-914685 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT106674 WP_000806442.1 NZ_CP029747:914721-915062 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT106674 NZ_CP029747:914721-915062 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References