Detailed information of TA system TA106645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5556086..5556681
• Replicon: chromosome
• Accession: NZ_CP029745
• Organism: Pseudomonas aeruginosa strain AR_0110

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: AM489_RS26470
• Protein ID: WP_003113526.1
• Coordinates: 5556403..5556681 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM489_RS26465
• Protein ID: WP_003113527.1
• Coordinates: 5556086..5556391 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM489_RS26445	5551793..5552584	+	792	WP_034019065.1	IS21-like element helper ATPase IstB	-
AM489_RS26450	5552791..5553654	-	864	WP_034019067.1	integrase domain-containing protein	-
AM489_RS26455	5554257..5555414	-	1158	WP_034019070.1	hypothetical protein	-
AM489_RS26465	5556086..5556391	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
AM489_RS26470	5556403..5556681	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM489_RS26480	5557010..5559238	+	2229	WP_033942306.1	TonB-dependent receptor	-
AM489_RS26485	5559308..5559955	-	648	WP_003095021.1	carbonate dehydratase	-
AM489_RS26490	5560017..5561255	-	1239	WP_033942304.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T106645 WP_003113526.1 NZ_CP029745:c5556681-5556403 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T106645 NZ_CP029745:c5556681-5556403 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT106645 WP_003113527.1 NZ_CP029745:c5556391-5556086 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT106645 NZ_CP029745:c5556391-5556086 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3