Detailed information of TA system TA106634

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26533..26959
• Replicon: plasmid unnamed3
• Accession: NZ_CP029744
• Organism: Escherichia coli strain AR_0085

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM464_RS29110
• Protein ID: WP_001312861.1
• Coordinates: 26533..26691 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26735..26959 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM464_RS29070	21903..22592	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
AM464_RS29075	22779..23162	-	384	WP_001151524.1	relaxosome protein TraM	-
AM464_RS29080	23483..24085	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
AM464_RS29085	24382..25203	-	822	WP_001234471.1	DUF945 domain-containing protein	-
AM464_RS29090	25324..25611	-	288	WP_000107535.1	hypothetical protein	-
AM464_RS30385	25636..25842	-	207	WP_000547939.1	hypothetical protein	-
AM464_RS30165	25955..26179	+	225	WP_077248521.1	hypothetical protein	-
AM464_RS29110	26533..26691	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	26735..26959	-	225	NuclAT_0	-	Antitoxin
-	26735..26959	-	225	NuclAT_0	-	Antitoxin
-	26735..26959	-	225	NuclAT_0	-	Antitoxin
-	26735..26959	-	225	NuclAT_0	-	Antitoxin
AM464_RS30170	26771..26959	+	189	WP_001299721.1	hypothetical protein	-
AM464_RS29115	26971..27690	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
AM464_RS29120	27687..28121	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
AM464_RS29125	28176..30134	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
AM464_RS29130	30193..30426	-	234	WP_000006004.1	DUF905 domain-containing protein	-
AM464_RS29135	30482..31009	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
AM464_RS29150	31354..31578	+	225	WP_001340580.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / tet(B)	iroB / iroC / iroD / iroE / iroN	1..117192	117192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T106634 WP_001312861.1 NZ_CP029744:c26691-26533 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T106634 NZ_CP029744:c26691-26533 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT106634 NZ_CP029744:c26959-26735 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin