Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 63599..64256 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP029743 | ||
| Organism | Escherichia coli strain AR_0085 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | AM464_RS28640 | Protein ID | WP_000270043.1 |
| Coordinates | 63906..64256 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | AM464_RS28635 | Protein ID | WP_000124640.1 |
| Coordinates | 63599..63901 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM464_RS28585 | 59224..59652 | + | 429 | WP_000591074.1 | hypothetical protein | - |
| AM464_RS28590 | 59709..60068 | + | 360 | WP_000422768.1 | hypothetical protein | - |
| AM464_RS28595 | 60068..60514 | + | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| AM464_RS28600 | 60511..61029 | + | 519 | WP_000210756.1 | nitrite reductase | - |
| AM464_RS28605 | 61029..61259 | + | 231 | WP_000972663.1 | hypothetical protein | - |
| AM464_RS28610 | 61246..62103 | + | 858 | WP_001167032.1 | hypothetical protein | - |
| AM464_RS28615 | 62129..62317 | + | 189 | WP_001270411.1 | hypothetical protein | - |
| AM464_RS28620 | 62334..62861 | + | 528 | WP_001236377.1 | thermonuclease family protein | - |
| AM464_RS28625 | 62919..63191 | + | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| AM464_RS28630 | 63279..63572 | + | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| AM464_RS28635 | 63599..63901 | - | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| AM464_RS28640 | 63906..64256 | - | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| AM464_RS28645 | 64419..64967 | + | 549 | WP_001061574.1 | transcriptional regulator | - |
| AM464_RS28655 | 65308..65502 | + | 195 | WP_000343597.1 | hypothetical protein | - |
| AM464_RS28660 | 65513..65884 | + | 372 | WP_000516916.1 | hypothetical protein | - |
| AM464_RS28665 | 65877..66347 | + | 471 | WP_001281821.1 | hypothetical protein | - |
| AM464_RS28670 | 66362..66697 | - | 336 | WP_000683476.1 | hypothetical protein | - |
| AM464_RS28675 | 66794..67282 | + | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| AM464_RS28680 | 67285..67782 | + | 498 | WP_000062185.1 | hypothetical protein | - |
| AM464_RS28685 | 67997..68701 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| AM464_RS28690 | 69008..69187 | + | 180 | Protein_86 | winged helix-turn-helix domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaCMY-2 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 | - | 1..104555 | 104555 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T106633 WP_000270043.1 NZ_CP029743:c64256-63906 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T106633 NZ_CP029743:c64256-63906 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT106633 WP_000124640.1 NZ_CP029743:c63901-63599 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT106633 NZ_CP029743:c63901-63599 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|