106613

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3475082..3475623 Replicon chromosome
Accession NZ_CP029741
Organism Escherichia coli strain AR_0085

Toxin (Protein)


Gene name yafQ Uniprot ID U9XF38
Locus tag AM464_RS17985 Protein ID WP_001695460.1
Coordinates 3475082..3475360 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AM464_RS17990 Protein ID WP_000729704.1
Coordinates 3475363..3475623 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM464_RS17970 3472664..3473242 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AM464_RS17975 3473448..3474215 + 768 WP_000333379.1 class II glutamine amidotransferase -
AM464_RS17980 3474186..3474926 - 741 WP_001225679.1 murein L,D-transpeptidase -
AM464_RS17985 3475082..3475360 - 279 WP_001695460.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AM464_RS17990 3475363..3475623 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AM464_RS17995 3475833..3476582 + 750 WP_001695461.1 C40 family peptidase -
AM464_RS18000 3476859..3477356 + 498 WP_000006265.1 REP-associated tyrosine transposase RayT -
AM464_RS18005 3477572..3479284 - 1713 Protein_3297 flagellar biosynthesis protein FlhA -
AM464_RS18010 3479256..3480041 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - vgrG/tssI / gmhA/lpcA 3457548..3477356 19808


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T106613 WP_001695460.1 NZ_CP029741:c3475360-3475082 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYIMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T106613 NZ_CP029741:c3475360-3475082 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATATTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT106613 WP_000729704.1 NZ_CP029741:c3475623-3475363 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT106613 NZ_CP029741:c3475623-3475363 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XF38

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References