Detailed information of TA system TA106599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1430186..1430879
• Replicon: chromosome
• Accession: NZ_CP029741
• Organism: Escherichia coli strain AR_0085

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: AM464_RS07365
• Protein ID: WP_000415584.1
• Coordinates: 1430583..1430879 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: AM464_RS07360
• Protein ID: WP_000650107.1
• Coordinates: 1430186..1430581 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM464_RS07345	1426050..1428308	-	2259	WP_110844015.1	DNA topoisomerase IV subunit A	-
AM464_RS07350	1428446..1430053	-	1608	WP_096282557.1	ABC transporter substrate-binding protein	-
AM464_RS07360	1430186..1430581	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
AM464_RS07365	1430583..1430879	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
AM464_RS07370	1431084..1431566	-	483	WP_000183505.1	transcriptional regulator	-
AM464_RS07375	1431619..1432011	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
AM464_RS07380	1432163..1432822	+	660	WP_001221493.1	two-component system response regulator QseB	-
AM464_RS07385	1432819..1434168	+	1350	WP_000673409.1	two-component system sensor histidine kinase QseC	-
AM464_RS07390	1434214..1434546	-	333	WP_000917684.1	DUF2645 family protein	-
AM464_RS07400	1434865..1435446	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
AM464_RS07405	1435477..1435791	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	dfrA1 / ant(3'')-Ia	kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / neuE / neuC / neuA / neuB / neuD / kpsT / kpsM / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC	1280860..1445877	165017

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T106599 WP_000415584.1 NZ_CP029741:c1430879-1430583 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T106599 NZ_CP029741:c1430879-1430583 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT106599 WP_000650107.1 NZ_CP029741:c1430581-1430186 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT106599 NZ_CP029741:c1430581-1430186 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65