Detailed information of TA system TA106593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 166747..167474
• Replicon: plasmid unnamed1
• Accession: NZ_CP029739
• Organism: Klebsiella pneumoniae strain AR_0087

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: AM466_RS28015
• Protein ID: WP_011251285.1
• Coordinates: 166747..167058 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM466_RS28020
• Protein ID: WP_011251286.1
• Coordinates: 167055..167474 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM466_RS27985	161978..162958	+	981	WP_048293850.1	IS5-like element ISKpn26 family transposase	-
AM466_RS27990	163503..163682	-	180	Protein_158	IS5/IS1182 family transposase	-
AM466_RS27995	163722..165260	-	1539	WP_032430751.1	IS66 family transposase	-
AM466_RS28000	165309..165656	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
AM466_RS28005	165653..165991	-	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
AM466_RS28010	166084..166542	+	459	Protein_162	DDE-type integrase/transposase/recombinase	-
AM466_RS28015	166747..167058	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
AM466_RS28020	167055..167474	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
AM466_RS28025	167645..168613	-	969	WP_016151349.1	IS5 family transposase	-
AM466_RS28030	168645..169856	-	1212	Protein_166	EstP	-
AM466_RS28035	169859..172066	-	2208	WP_048322244.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..175746	175746
	-	inside	IScluster/Tn	-	-	161978..175283	13305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T106593 WP_011251285.1 NZ_CP029739:166747-167058 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T106593 NZ_CP029739:166747-167058 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT106593 WP_011251286.1 NZ_CP029739:167055-167474 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT106593 NZ_CP029739:167055-167474 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCATATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1