Detailed information of TA system TA106574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 112826..113483
• Replicon: plasmid unnamed
• Accession: NZ_CP029737
• Organism: Providencia rettgeri strain AR_0082

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AM461_RS20655
• Protein ID: WP_000270043.1
• Coordinates: 112826..113176 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM461_RS20660
• Protein ID: WP_000124640.1
• Coordinates: 113181..113483 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM461_RS20615	109300..109797	-	498	WP_000062185.1	hypothetical protein	-
AM461_RS20620	109800..110288	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
AM461_RS20625	110385..110720	+	336	WP_000683477.1	hypothetical protein	-
AM461_RS20630	110735..111205	-	471	WP_001281821.1	hypothetical protein	-
AM461_RS20635	111198..111569	-	372	WP_000516918.1	hypothetical protein	-
AM461_RS20640	111580..111774	-	195	WP_000343597.1	hypothetical protein	-
AM461_RS20650	112115..112663	-	549	WP_001061573.1	hypothetical protein	-
AM461_RS20655	112826..113176	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM461_RS20660	113181..113483	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AM461_RS20665	113510..113803	-	294	WP_001239998.1	hypothetical protein	-
AM461_RS20670	113892..114164	-	273	WP_001043046.1	HU family DNA-binding protein	-
AM461_RS20675	114222..114749	-	528	WP_001210659.1	thermonuclease family protein	-
AM461_RS21115	114752..115057	-	306	WP_171820680.1	hypothetical protein	-
AM461_RS20685	114969..115268	-	300	Protein_140	hypothetical protein	-
AM461_RS20690	115490..117031	+	1542	WP_001274811.1	IS21-like element ISAs29 family transposase	-
AM461_RS20695	117046..117801	+	756	WP_000194037.1	IS21-like element ISAs29 family helper ATPase IstB	-
AM461_RS20700	117857..118444	-	588	Protein_143	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1 / sul1 / qacE / aadA2 / dfrA12	htpB	1..144970	144970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T106574 WP_000270043.1 NZ_CP029737:112826-113176 [Providencia rettgeri]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T106574 NZ_CP029737:112826-113176 [Providencia rettgeri]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT106574 WP_000124640.1 NZ_CP029737:113181-113483 [Providencia rettgeri]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT106574 NZ_CP029737:113181-113483 [Providencia rettgeri]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9