Detailed information of TA system TA106566

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 8009..8435
• Replicon: plasmid unnamed6
• Accession: NZ_CP029734
• Organism: Citrobacter sp. CRE-46 strain AR_0157

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AN232_RS31700
• Protein ID: WP_001312861.1
• Coordinates: 8009..8167 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	8211..8435 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN232_RS30400	3032..3259	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
AN232_RS30405	3396..4067	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
AN232_RS30410	4261..4644	-	384	WP_001354030.1	relaxosome protein TraM	-
AN232_RS30420	4967..5569	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
AN232_RS30425	5866..6687	-	822	WP_001234445.1	DUF945 domain-containing protein	-
AN232_RS30430	6798..7094	-	297	WP_001272251.1	hypothetical protein	-
AN232_RS31905	7394..7771	+	378	Protein_11	hypothetical protein	-
AN232_RS31700	8009..8167	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	8211..8435	-	225	NuclAT_0	-	Antitoxin
-	8211..8435	-	225	NuclAT_0	-	Antitoxin
-	8211..8435	-	225	NuclAT_0	-	Antitoxin
-	8211..8435	-	225	NuclAT_0	-	Antitoxin
AN232_RS30455	8447..9166	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
AN232_RS30460	9163..9597	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
AN232_RS30465	9666..11630	-	1965	WP_063131970.1	ParB/RepB/Spo0J family partition protein	-
AN232_RS30470	11691..11924	-	234	WP_000006003.1	DUF905 family protein	-
AN232_RS30475	11982..12509	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / mph(A) / erm(B)	-	1..76953	76953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T106566 WP_001312861.1 NZ_CP029734:c8167-8009 [Citrobacter sp. CRE-46]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T106566 NZ_CP029734:c8167-8009 [Citrobacter sp. CRE-46]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT106566 NZ_CP029734:c8435-8211 [Citrobacter sp. CRE-46]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin