Detailed information of TA system TA106563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 10684..11341
• Replicon: plasmid unnamed2
• Accession: NZ_CP029730
• Organism: Citrobacter sp. CRE-46 strain AR_0157

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AN232_RS28030
• Protein ID: WP_000270043.1
• Coordinates: 10684..11034 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AN232_RS28035
• Protein ID: WP_000124640.1
• Coordinates: 11039..11341 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN232_RS27990	7158..7655	-	498	WP_000062185.1	hypothetical protein	-
AN232_RS27995	7658..8146	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
AN232_RS28000	8243..8578	+	336	WP_000683477.1	hypothetical protein	-
AN232_RS28005	8593..9063	-	471	WP_001281821.1	hypothetical protein	-
AN232_RS28010	9056..9427	-	372	WP_000516918.1	hypothetical protein	-
AN232_RS28015	9438..9632	-	195	WP_000343597.1	hypothetical protein	-
AN232_RS28025	9973..10521	-	549	WP_001061573.1	hypothetical protein	-
AN232_RS28030	10684..11034	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AN232_RS28035	11039..11341	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AN232_RS28040	11368..11661	-	294	WP_001239998.1	hypothetical protein	-
AN232_RS28045	11750..12022	-	273	WP_001043046.1	HU family DNA-binding protein	-
AN232_RS28050	12080..12607	-	528	WP_001210659.1	thermonuclease family protein	-
AN232_RS28055	12802..14001	+	1200	WP_110498863.1	site-specific integrase	-
AN232_RS28060	13998..15137	+	1140	WP_110498864.1	DUF1016 family protein	-
AN232_RS28065	15372..15695	+	324	WP_110498865.1	HigA family addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..136246	136246

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T106563 WP_000270043.1 NZ_CP029730:10684-11034 [Citrobacter sp. CRE-46]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T106563 NZ_CP029730:10684-11034 [Citrobacter sp. CRE-46]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT106563 WP_000124640.1 NZ_CP029730:11039-11341 [Citrobacter sp. CRE-46]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT106563 NZ_CP029730:11039-11341 [Citrobacter sp. CRE-46]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9