Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 466456..467120 Replicon chromosome
Accession NZ_CP029727
Organism Citrobacter sp. CRE-46 strain AR_0157

Toxin (Protein)


Gene name higB Uniprot ID A0A2Z3X092
Locus tag AN232_RS02375 Protein ID WP_038636359.1
Coordinates 466818..467120 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2Z3X2H4
Locus tag AN232_RS02370 Protein ID WP_038636361.1
Coordinates 466456..466797 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN232_RS02340 461980..462738 + 759 WP_038636380.1 phosphonate C-P lyase system protein PhnK -
AN232_RS02345 462846..463526 + 681 WP_042312926.1 phosphonate C-P lyase system protein PhnL -
AN232_RS02350 463523..464659 + 1137 WP_038636374.1 alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase -
AN232_RS02355 464662..465216 + 555 WP_110496205.1 ribose 1,5-bisphosphokinase -
AN232_RS02360 465203..465637 + 435 WP_069325128.1 aminoalkylphosphonate N-acetyltransferase -
AN232_RS02365 465646..466404 + 759 WP_110496207.1 phosphonate metabolism protein PhnP -
AN232_RS02370 466456..466797 - 342 WP_038636361.1 HigA family addiction module antidote protein Antitoxin
AN232_RS02375 466818..467120 - 303 WP_038636359.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AN232_RS02380 467276..467605 - 330 WP_110496209.1 hypothetical protein -
AN232_RS02385 467675..469939 - 2265 WP_110496211.1 hybrid sensor histidine kinase/response regulator -
AN232_RS02395 470054..471577 + 1524 WP_069325124.1 sugar ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11856.50 Da        Isoelectric Point: 9.9581

>T106546 WP_038636359.1 NZ_CP029727:c467120-466818 [Citrobacter sp. CRE-46]
MSKSLNIRNFRDTWLEDFFERATPHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Download         Length: 303 bp

>T106546 NZ_CP029727:c467120-466818 [Citrobacter sp. CRE-46]
ATGTCTAAATCATTAAATATCAGAAATTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCAACACCGCACAGAAA
AATACCCGCGGATATTCACACCGCATTAGCCAGAAAGCTGGATATCATCAATGCGGCGGTTTCGCATCGGGATTTGCGCT
CGCCGCCAGGGAATAGATATGAAGAGTTAACCGGAAAGTTACAGGAATATTCTTCAATAAGAGTAAATAAGCAGTACCGA
TTAATATTTAAATGGGTTAACGGTAAGGCCGAAGATGTGTACTTAGACCCACACATCTACTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13063.93 Da        Isoelectric Point: 7.2038

>AT106546 WP_038636361.1 NZ_CP029727:c466797-466456 [Citrobacter sp. CRE-46]
MKQATRKPTTVGDVLQYEYLEPLDLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQSAVDLW
EVENDMRAQEELNRIMSVKDFVAQRNVATKKVA

Download         Length: 342 bp

>AT106546 NZ_CP029727:c466797-466456 [Citrobacter sp. CRE-46]
ATGAAACAGGCAACCAGAAAACCAACTACTGTGGGTGATGTCCTGCAATATGAATACCTGGAGCCGCTCGATCTGAAAAT
TAACGATCTAGCTGAAATACTGCATGTTCACCGCAATACGGTGAGCGCGCTGGTCAATAATAATCGCAAATTAACAACGG
ATATGGCATTTCGCCTGGCGAAAGCGTTTGATACCTCCGTTGATTTCTGGCGTAACCTACAGTCGGCGGTTGACCTGTGG
GAAGTTGAAAATGATATGCGGGCGCAAGAAGAACTGAACCGTATTATGTCTGTTAAAGACTTTGTCGCGCAGCGAAATGT
CGCCACTAAGAAAGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3X092

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3X2H4

References