Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 41324..41981 | Replicon | plasmid unnamed5 |
| Accession | NZ_CP029718 | ||
| Organism | Enterobacter cloacae complex sp. strain AR_0154 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | AM401_RS25415 | Protein ID | WP_000270043.1 |
| Coordinates | 41324..41674 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | AM401_RS25420 | Protein ID | WP_000124640.1 |
| Coordinates | 41679..41981 (+) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM401_RS25375 (AM401_25370) | 37798..38295 | - | 498 | WP_000062185.1 | hypothetical protein | - |
| AM401_RS25380 (AM401_25375) | 38298..38786 | - | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| AM401_RS25385 (AM401_25380) | 38883..39218 | + | 336 | WP_000683477.1 | hypothetical protein | - |
| AM401_RS25390 (AM401_25385) | 39233..39703 | - | 471 | WP_001281821.1 | hypothetical protein | - |
| AM401_RS25395 (AM401_25390) | 39696..40067 | - | 372 | WP_000516918.1 | hypothetical protein | - |
| AM401_RS25400 (AM401_25395) | 40078..40272 | - | 195 | WP_000343597.1 | hypothetical protein | - |
| AM401_RS25410 (AM401_25405) | 40613..41161 | - | 549 | WP_001061573.1 | hypothetical protein | - |
| AM401_RS25415 (AM401_25410) | 41324..41674 | + | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| AM401_RS25420 (AM401_25415) | 41679..41981 | + | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| AM401_RS25425 (AM401_25420) | 42008..42301 | - | 294 | WP_001239998.1 | hypothetical protein | - |
| AM401_RS25430 (AM401_25425) | 42390..42662 | - | 273 | WP_001043046.1 | HU family DNA-binding protein | - |
| AM401_RS25435 (AM401_25430) | 42720..43247 | - | 528 | WP_004201083.1 | thermonuclease family protein | - |
| AM401_RS28095 (AM401_25435) | 43250..43441 | - | 192 | WP_001270409.1 | hypothetical protein | - |
| AM401_RS25445 (AM401_25440) | 43467..44324 | - | 858 | WP_001167036.1 | hypothetical protein | - |
| AM401_RS25450 (AM401_25445) | 44311..44541 | - | 231 | WP_000972665.1 | hypothetical protein | - |
| AM401_RS25455 (AM401_25450) | 44541..45059 | - | 519 | WP_000210757.1 | nitrite reductase | - |
| AM401_RS25460 (AM401_25455) | 45056..45502 | - | 447 | WP_000919343.1 | hypothetical protein | - |
| AM401_RS25465 (AM401_25460) | 45502..45861 | - | 360 | WP_000422769.1 | hypothetical protein | - |
| AM401_RS25470 (AM401_25465) | 45919..46347 | - | 429 | WP_000591076.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | dfrA12 / aadA2 / qacE / sul1 / qnrA1 / mph(A) / ant(3'')-Ia / dfrA1 / aac(6')-Il / blaVIM-1 | - | 1..158987 | 158987 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T106516 WP_000270043.1 NZ_CP029718:41324-41674 [Enterobacter cloacae complex sp.]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T106516 NZ_CP029718:41324-41674 [Enterobacter cloacae complex sp.]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT106516 WP_000124640.1 NZ_CP029718:41679-41981 [Enterobacter cloacae complex sp.]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT106516 NZ_CP029718:41679-41981 [Enterobacter cloacae complex sp.]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|