Detailed information of TA system TA106477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4393375..4393970
• Replicon: chromosome
• Accession: NZ_CP029707
• Organism: Pseudomonas aeruginosa strain K34-7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: DCS61_RS21295
• Protein ID: WP_003113526.1
• Coordinates: 4393692..4393970 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DCS61_RS21290
• Protein ID: WP_003113527.1
• Coordinates: 4393375..4393680 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCS61_RS21255	4388526..4389374	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
DCS61_RS21265	4389541..4390482	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
DCS61_RS21270	4390599..4391213	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
DCS61_RS21275	4391255..4391839	+	585	WP_033942324.1	aminoacyl-tRNA hydrolase	-
DCS61_RS21280	4391880..4392980	+	1101	WP_033942321.1	redox-regulated ATPase YchF	-
DCS61_RS21290	4393375..4393680	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
DCS61_RS21295	4393692..4393970	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DCS61_RS21305	4394299..4396527	+	2229	WP_033942306.1	TonB-dependent receptor	-
DCS61_RS21310	4396597..4397244	-	648	WP_003095021.1	carbonate dehydratase	-
DCS61_RS21315	4397306..4398544	-	1239	WP_033942304.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T106477 WP_003113526.1 NZ_CP029707:c4393970-4393692 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T106477 NZ_CP029707:c4393970-4393692 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT106477 WP_003113527.1 NZ_CP029707:c4393680-4393375 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT106477 NZ_CP029707:c4393680-4393375 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3