Detailed information of TA system TA106465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1920846..1921500
• Replicon: chromosome
• Accession: NZ_CP029693
• Organism: Pseudomonas putida strain JBC17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DKY63_RS08745
• Protein ID: WP_110963745.1
• Coordinates: 1920846..1921196 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DKY63_RS08750
• Protein ID: WP_110963746.1
• Coordinates: 1921186..1921500 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DKY63_RS08735	1917593..1919125	+	1533	WP_110963743.1	NADH-quinone oxidoreductase subunit M	-
DKY63_RS08740	1919133..1920596	+	1464	WP_110963744.1	NADH-quinone oxidoreductase subunit NuoN	-
DKY63_RS08745	1920846..1921196	+	351	WP_110963745.1	toxin	Toxin
DKY63_RS08750	1921186..1921500	+	315	WP_110963746.1	transcriptional regulator	Antitoxin
DKY63_RS08755	1921728..1923470	-	1743	WP_110963747.1	ABC transporter substrate-binding protein	-
DKY63_RS08760	1923518..1923790	-	273	WP_110963748.1	DUF2160 domain-containing protein	-
DKY63_RS08765	1923801..1924601	-	801	WP_110963749.1	carbohydrate ABC transporter permease	-
DKY63_RS08770	1924610..1925476	-	867	WP_110963750.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13958.80 Da
• Isoelectric Point: 8.0097

>T106465 WP_110963745.1 NZ_CP029693:1920846-1921196 [Pseudomonas putida]
MDALFIELPLFQKHRDDYLDDDLFRSFQLELLQNPEAGDLIEGTGGLRKIRFCDQRRGKGKRSGLRVIYYWWSGFNQFWL
FTVYGKNEQDDLSPFQKKVFRQALDREIKTRSHYET

Nucleotide

• Length: 351 bp

>T106465 NZ_CP029693:1920846-1921196 [Pseudomonas putida]
ATGGATGCTCTGTTCATTGAATTACCTTTGTTTCAAAAACATCGAGACGATTATCTGGACGACGATTTGTTTCGCAGCTT
TCAGTTGGAGTTGCTGCAGAACCCCGAAGCAGGCGATCTCATTGAGGGAACCGGCGGGCTGCGAAAAATTCGCTTCTGCG
ACCAACGAAGAGGTAAAGGCAAGCGCAGTGGCTTACGAGTAATTTACTACTGGTGGTCCGGCTTCAATCAATTCTGGCTG
TTCACTGTGTACGGCAAAAACGAGCAAGACGACCTGTCGCCCTTCCAGAAGAAAGTTTTCAGACAAGCACTGGATAGAGA
AATCAAAACGAGATCCCACTATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11970.85 Da
• Isoelectric Point: 7.0150

>AT106465 WP_110963746.1 NZ_CP029693:1921186-1921500 [Pseudomonas putida]
MKRDIFAELMDGLEALADERQGKITLRTHKVQLPELMPITAEEVIAIRQQLNLSRSVFAMYLRTNTRTLENWEQGRATPN
AQATTLIRLVERFPQTIEYLAALT

Nucleotide

• Length: 315 bp

>AT106465 NZ_CP029693:1921186-1921500 [Pseudomonas putida]
ATGAAACGTGACATTTTTGCCGAACTGATGGATGGCCTCGAAGCCCTGGCCGATGAGCGCCAGGGCAAGATCACGCTGCG
AACCCACAAGGTTCAGTTGCCAGAACTGATGCCCATCACCGCCGAGGAAGTAATCGCCATCCGCCAACAGCTCAACCTCT
CCCGGTCGGTGTTTGCGATGTATCTGCGTACCAACACCCGAACACTGGAGAACTGGGAACAGGGGCGCGCAACGCCCAAT
GCCCAAGCCACGACTCTGATCCGTCTGGTCGAGCGTTTTCCACAGACGATCGAATATCTCGCAGCCCTGACCTGA