Detailed information of TA system TA106459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4749200..4749802
• Replicon: chromosome
• Accession: NZ_CP029692
• Organism: Escherichia coli strain SD134209

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: DL800_RS27120
• Protein ID: WP_000897305.1
• Coordinates: 4749200..4749511 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DL800_RS27125
• Protein ID: WP_000356397.1
• Coordinates: 4749512..4749802 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DL800_RS27090	4744230..4745015	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
DL800_RS27095	4745114..4745713	+	600	WP_001315111.1	glucose-1-phosphatase	-
DL800_RS27100	4745707..4746579	+	873	WP_000920762.1	virulence factor BrkB family protein	-
DL800_RS27105	4746576..4747013	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DL800_RS27110	4747058..4747999	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DL800_RS27115	4748063..4748971	-	909	WP_001162704.1	alpha/beta hydrolase	-
DL800_RS27120	4749200..4749511	+	312	WP_000897305.1	hypothetical protein	Toxin
DL800_RS27125	4749512..4749802	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
DL800_RS27135	4750407..4750625	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
DL800_RS27140	4750844..4751086	+	243	WP_001086388.1	hypothetical protein	-
DL800_RS27145	4751417..4752346	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
DL800_RS27150	4752343..4752978	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
DL800_RS27155	4752975..4753877	-	903	WP_000331369.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T106459 WP_000897305.1 NZ_CP029692:4749200-4749511 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T106459 NZ_CP029692:4749200-4749511 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT106459 WP_000356397.1 NZ_CP029692:4749512-4749802 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT106459 NZ_CP029692:4749512-4749802 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7