Detailed information of TA system TA106435

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27168..27437
• Replicon: plasmid pSD134209-2
• Accession: NZ_CP029691
• Organism: Escherichia coli strain SD134209

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DL800_RS01320
• Protein ID: WP_001312861.1
• Coordinates: 27279..27437 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27168..27233 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DL800_RS01275	22228..22488	+	261	WP_071600428.1	hypothetical protein	-
DL800_RS01295	22961..23488	+	528	WP_000290792.1	single-stranded DNA-binding protein	-
DL800_RS01300	23544..23777	+	234	WP_000006004.1	DUF905 domain-containing protein	-
DL800_RS01305	23836..25794	+	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
DL800_RS01310	25849..26283	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
DL800_RS01315	26280..26999	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
DL800_RS30360	27011..27199	-	189	WP_001299721.1	hypothetical protein	-
-	27011..27235	+	225	NuclAT_0	-	-
-	27011..27235	+	225	NuclAT_0	-	-
-	27011..27235	+	225	NuclAT_0	-	-
-	27011..27235	+	225	NuclAT_0	-	-
-	27168..27233	+	66	-	-	Antitoxin
DL800_RS01320	27279..27437	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
DL800_RS30365	27791..28015	-	225	WP_001427866.1	hypothetical protein	-
DL800_RS01340	28359..28646	+	288	WP_000107535.1	hypothetical protein	-
DL800_RS01345	28767..29588	+	822	WP_001234469.1	DUF945 domain-containing protein	-
DL800_RS01350	29885..30394	-	510	Protein_37	transglycosylase SLT domain-containing protein	-
DL800_RS01355	30808..31191	+	384	WP_001151524.1	relaxosome protein TraM	-
DL800_RS01360	31378..32067	+	690	WP_000283394.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyD / hlyB / hlyA / hlyC	1..78329	78329

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T106435 WP_001312861.1 NZ_CP029691:27279-27437 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T106435 NZ_CP029691:27279-27437 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT106435 NZ_CP029691:27168-27233 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin