Detailed information of TA system TA106410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3694209..3694903
• Replicon: chromosome
• Accession: NZ_CP029687
• Organism: Escherichia coli strain E706

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: DMI84_RS19820
• Protein ID: WP_001263493.1
• Coordinates: 3694209..3694607 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: DMI84_RS19825
• Protein ID: WP_000554757.1
• Coordinates: 3694610..3694903 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3689750..3689830	-	81	NuclAT_11	-	-
-	3689750..3689830	-	81	NuclAT_11	-	-
-	3689750..3689830	-	81	NuclAT_11	-	-
-	3689750..3689830	-	81	NuclAT_11	-	-
DMI84_RS19785	3690426..3690884	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
DMI84_RS19790	3691145..3692602	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
DMI84_RS19795	3692659..3693180	-	522	Protein_3616	peptide chain release factor H	-
DMI84_RS19800	3693179..3693373	-	195	Protein_3617	RtcB family protein	-
DMI84_RS19810	3693423..3694120	+	698	Protein_3618	IS1 family transposase	-
DMI84_RS19815	3694137..3694199	-	63	Protein_3619	GNAT family N-acetyltransferase	-
DMI84_RS19820	3694209..3694607	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DMI84_RS19825	3694610..3694903	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DMI84_RS19830	3694955..3696009	-	1055	Protein_3622	DNA polymerase IV	-
DMI84_RS19835	3696080..3697003	-	924	WP_001468020.1	putative lateral flagellar export/assembly protein LafU	-
DMI84_RS19840	3697006..3697869	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
DMI84_RS19845	3697882..3698601	-	720	WP_000938740.1	FliA/WhiG family RNA polymerase sigma factor	-
DMI84_RS19850	3698621..3699088	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3693743..3694120	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T106410 WP_001263493.1 NZ_CP029687:c3694607-3694209 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T106410 NZ_CP029687:c3694607-3694209 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT106410 WP_000554757.1 NZ_CP029687:c3694903-3694610 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT106410 NZ_CP029687:c3694903-3694610 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6