Detailed information of TA system TA106167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4077788..4078383
• Replicon: chromosome
• Accession: NZ_CP029660
• Organism: Pseudomonas aeruginosa strain AR_0446

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CSC33_RS19495
• Protein ID: WP_003113526.1
• Coordinates: 4078105..4078383 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSC33_RS19490
• Protein ID: WP_033971055.1
• Coordinates: 4077788..4078093 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC33_RS19455	4072938..4073786	+	849	WP_033971048.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
CSC33_RS19465	4073953..4074894	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CSC33_RS19470	4075011..4075625	+	615	WP_033971050.1	50S ribosomal protein L25/general stress protein Ctc	-
CSC33_RS19475	4075667..4076251	+	585	WP_033971054.1	aminoacyl-tRNA hydrolase	-
CSC33_RS19480	4076292..4077392	+	1101	WP_058178912.1	redox-regulated ATPase YchF	-
CSC33_RS19490	4077788..4078093	-	306	WP_033971055.1	HigA family addiction module antidote protein	Antitoxin
CSC33_RS19495	4078105..4078383	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC33_RS19500	4078436..4078897	-	462	Protein_3767	integrase	-
CSC33_RS19505	4078901..4079320	-	420	WP_033971056.1	type II toxin-antitoxin system VapC family toxin	-
CSC33_RS19510	4079317..4079562	-	246	WP_033971057.1	Arc family DNA-binding protein	-
CSC33_RS19515	4079588..4079826	-	239	Protein_3770	tyrosine-type recombinase/integrase	-
CSC33_RS19520	4080195..4080398	+	204	WP_033971061.1	hypothetical protein	-
CSC33_RS31160	4080649..4080945	+	297	WP_162601966.1	hypothetical protein	-
CSC33_RS19525	4080995..4082157	+	1163	WP_108089267.1	IS3 family transposase	-
CSC33_RS19530	4082209..4082859	-	651	WP_033971066.1	carbonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T106167 WP_003113526.1 NZ_CP029660:c4078383-4078105 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T106167 NZ_CP029660:c4078383-4078105 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCCATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11230.69 Da
• Isoelectric Point: 4.4462

>AT106167 WP_033971055.1 NZ_CP029660:c4078093-4077788 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPTALARALNVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT106167 NZ_CP029660:c4078093-4077788 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AACTGCTCTAGCACGCGCTTTGAACGTCTCCGCCCCGACAGTGAACGATATTGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGACACGTCCGCTCAGTTCTGGATGAATCTTCAGAGTGAGTATTCGTTGGCA
ACTGCTTATGCGGCGAACGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3