Detailed information of TA system TA106001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6007617..6008212
• Replicon: chromosome
• Accession: NZ_CP029605
• Organism: Pseudomonas aeruginosa strain 24Pae112

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CTT40_RS29155
• Protein ID: WP_003113526.1
• Coordinates: 6007934..6008212 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CTT40_RS29150
• Protein ID: WP_003133769.1
• Coordinates: 6007617..6007922 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTT40_RS29115	6002768..6003616	+	849	WP_003095007.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
CTT40_RS29125	6003783..6004724	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CTT40_RS29130	6004841..6005455	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
CTT40_RS29135	6005497..6006081	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
CTT40_RS29140	6006122..6007222	+	1101	WP_003095017.1	redox-regulated ATPase YchF	-
CTT40_RS29150	6007617..6007922	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
CTT40_RS29155	6007934..6008212	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CTT40_RS29165	6008541..6010769	+	2229	WP_023104079.1	TonB-dependent receptor	-
CTT40_RS29170	6010840..6011487	-	648	WP_003095021.1	carbonate dehydratase	-
CTT40_RS29175	6011549..6012787	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T106001 WP_003113526.1 NZ_CP029605:c6008212-6007934 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T106001 NZ_CP029605:c6008212-6007934 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT106001 WP_003133769.1 NZ_CP029605:c6007922-6007617 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT106001 NZ_CP029605:c6007922-6007617 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3