Detailed information of TA system TA105937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2415397..2416037
• Replicon: chromosome
• Accession: NZ_CP029587
• Organism: Klebsiella pneumoniae strain DA33141

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: DLJ66_RS11965
• Protein ID: WP_016529833.1
• Coordinates: 2415397..2415744 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DLJ66_RS11970
• Protein ID: WP_040181955.1
• Coordinates: 2415744..2416037 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ66_RS11955	2411323..2412756	+	1434	WP_002920564.1	glycogen synthase GlgA	-
DLJ66_RS11960	2412774..2415221	+	2448	WP_002920561.1	glycogen phosphorylase	-
DLJ66_RS11965	2415397..2415744	+	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DLJ66_RS11970	2415744..2416037	+	294	WP_040181955.1	helix-turn-helix transcriptional regulator	Antitoxin
DLJ66_RS11975	2416107..2417615	-	1509	WP_023301456.1	glycerol-3-phosphate dehydrogenase	-
DLJ66_RS11980	2417820..2418149	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
DLJ66_RS11985	2418200..2419030	+	831	WP_024622927.1	rhomboid family intramembrane serine protease GlpG	-
DLJ66_RS11990	2419080..2419838	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T105937 WP_016529833.1 NZ_CP029587:2415397-2415744 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T105937 NZ_CP029587:2415397-2415744 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10539.30 Da
• Isoelectric Point: 10.1464

>AT105937 WP_040181955.1 NZ_CP029587:2415744-2416037 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKSLV

Nucleotide

• Length: 294 bp

>AT105937 NZ_CP029587:2415744-2416037 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAAGTCTCGTTTAA

Structures

Toxin

• AlphaFold DB: W9BBY1