Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 1699674..1700260 Replicon chromosome
Accession NZ_CP029582
Organism Klebsiella pneumoniae strain DA33140

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag DLJ83_RS08460 Protein ID WP_002920800.1
Coordinates 1699674..1700042 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag DLJ83_RS08465 Protein ID WP_004174006.1
Coordinates 1700039..1700260 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ83_RS08440 1695177..1696247 - 1071 WP_032433652.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
DLJ83_RS08445 1696249..1697094 - 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
DLJ83_RS08450 1697091..1697978 - 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
DLJ83_RS08455 1698085..1699401 - 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
DLJ83_RS08460 1699674..1700042 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DLJ83_RS08465 1700039..1700260 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DLJ83_RS08470 1700424..1701137 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
DLJ83_RS08475 1701139..1701906 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
DLJ83_RS08480 1701903..1703180 - 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
DLJ83_RS08485 1703177..1704103 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1694440..1715399 20959


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T105914 WP_002920800.1 NZ_CP029582:c1700042-1699674 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T105914 NZ_CP029582:c1700042-1699674 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT105914 WP_004174006.1 NZ_CP029582:c1700260-1700039 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT105914 NZ_CP029582:c1700260-1700039 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References