Detailed information of TA system TA105906

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 151188..151614
• Replicon: plasmid pDA33137-178
• Accession: NZ_CP029580
• Organism: Escherichia coli strain DA33137

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DLJ64_RS28230
• Protein ID: WP_001312861.1
• Coordinates: 151188..151346 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	151390..151614 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ64_RS28185	146300..146527	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
DLJ64_RS28190	146615..147292	-	678	WP_001348626.1	PAS domain-containing protein	-
DLJ64_RS28195	147426..147809	-	384	WP_001151566.1	relaxosome protein TraM	-
DLJ64_RS28200	148140..148742	+	603	WP_110074666.1	transglycosylase SLT domain-containing protein	-
DLJ64_RS28205	149039..149860	-	822	WP_001234469.1	DUF945 domain-containing protein	-
DLJ64_RS28210	149979..150266	-	288	WP_000107535.1	hypothetical protein	-
DLJ64_RS28985	150291..150497	-	207	WP_000275859.1	hypothetical protein	-
DLJ64_RS28230	151188..151346	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	151390..151614	-	225	NuclAT_0	-	Antitoxin
-	151390..151614	-	225	NuclAT_0	-	Antitoxin
-	151390..151614	-	225	NuclAT_0	-	Antitoxin
-	151390..151614	-	225	NuclAT_0	-	Antitoxin
DLJ64_RS28785	151426..151614	+	189	WP_001299721.1	hypothetical protein	-
DLJ64_RS28235	151626..152345	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
DLJ64_RS28240	152342..152776	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
DLJ64_RS28245	152831..154789	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
DLJ64_RS28250	154855..155088	-	234	WP_000005990.1	DUF905 family protein	-
DLJ64_RS28255	155151..155690	-	540	WP_000290841.1	single-stranded DNA-binding protein	-
DLJ64_RS28790	156333..156575	-	243	WP_001617878.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qacE / blaCTX-M-14 / aadA5 / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / cmlA1	senB	1..178078	178078
	-	inside	Integron	-	senB	27..178076	178049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T105906 WP_001312861.1 NZ_CP029580:c151346-151188 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T105906 NZ_CP029580:c151346-151188 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT105906 NZ_CP029580:c151614-151390 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin