Detailed information of TA system TA105885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2427723..2428325
• Replicon: chromosome
• Accession: NZ_CP029579
• Organism: Escherichia coli strain DA33137

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: DLJ64_RS12640
• Protein ID: WP_000897305.1
• Coordinates: 2428014..2428325 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DLJ64_RS12635
• Protein ID: WP_000356395.1
• Coordinates: 2427723..2428013 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ64_RS12595	2423346..2424248	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
DLJ64_RS12600	2424245..2424880	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
DLJ64_RS12605	2424877..2425806	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
DLJ64_RS12610	2425988..2426230	-	243	WP_021523315.1	hypothetical protein	-
DLJ64_RS12615	2426449..2426667	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
DLJ64_RS12625	2427086..2427364	-	279	WP_001306650.1	hypothetical protein	-
DLJ64_RS12630	2427426..2427638	-	213	WP_000197769.1	hypothetical protein	-
DLJ64_RS12635	2427723..2428013	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
DLJ64_RS12640	2428014..2428325	-	312	WP_000897305.1	hypothetical protein	Toxin
DLJ64_RS12645	2428554..2429462	+	909	WP_001306651.1	alpha/beta hydrolase	-
DLJ64_RS12655	2429631..2431445	-	1815	WP_020233236.1	hypothetical protein	-
DLJ64_RS12660	2431861..2432802	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DLJ64_RS12665	2432847..2433284	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T105885 WP_000897305.1 NZ_CP029579:c2428325-2428014 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T105885 NZ_CP029579:c2428325-2428014 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT105885 WP_000356395.1 NZ_CP029579:c2428013-2427723 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT105885 NZ_CP029579:c2428013-2427723 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7