Detailed information of TA system TA105879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1505048..1505742
• Replicon: chromosome
• Accession: NZ_CP029579
• Organism: Escherichia coli strain DA33137

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: DLJ64_RS07870
• Protein ID: WP_001521903.1
• Coordinates: 1505048..1505446 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: DLJ64_RS07875
• Protein ID: WP_000554758.1
• Coordinates: 1505449..1505742 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ64_RS07840	1500218..1501462	-	1245	WP_000189541.1	esterase FrsA	-
-	1500878..1500958	-	81	NuclAT_11	-	-
-	1500878..1500958	-	81	NuclAT_11	-	-
-	1500878..1500958	-	81	NuclAT_11	-	-
-	1500878..1500958	-	81	NuclAT_11	-	-
DLJ64_RS07845	1501554..1502012	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
DLJ64_RS07850	1502273..1503730	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
DLJ64_RS07855	1503787..1504139	-	353	Protein_1427	peptide chain release factor H	-
DLJ64_RS07860	1504135..1504341	-	207	Protein_1428	RtcB family protein	-
DLJ64_RS07865	1504586..1505038	-	453	WP_023144376.1	GNAT family N-acetyltransferase	-
DLJ64_RS07870	1505048..1505446	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DLJ64_RS07875	1505449..1505742	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DLJ64_RS07880	1505794..1506849	-	1056	WP_001226177.1	DNA polymerase IV	-
DLJ64_RS07885	1506920..1507705	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
DLJ64_RS07890	1507677..1509389	+	1713	Protein_1434	flagellar biosynthesis protein FlhA	-
DLJ64_RS07895	1509468..1509821	+	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
DLJ64_RS07900	1509878..1510627	-	750	WP_016233951.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1494985..1505742	10757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T105879 WP_001521903.1 NZ_CP029579:c1505446-1505048 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T105879 NZ_CP029579:c1505446-1505048 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT105879 WP_000554758.1 NZ_CP029579:c1505742-1505449 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT105879 NZ_CP029579:c1505742-1505449 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3