Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 614491..614993 Replicon chromosome
Accession NZ_CP029579
Organism Escherichia coli strain DA33137

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DLJ64_RS03440 Protein ID WP_000767829.1
Coordinates 614491..614745 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DLJ64_RS03445 Protein ID WP_001259255.1
Coordinates 614742..614993 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ64_RS03410 609506..609733 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DLJ64_RS03415 609747..610805 - 1059 WP_000492324.1 transport protein YeeE -
DLJ64_RS03420 610984..612342 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DLJ64_RS03425 612332..612394 - 63 WP_010723108.1 membrane protein YoeI -
DLJ64_RS03430 612609..613538 - 930 WP_023154044.1 LysR family transcriptional regulator -
DLJ64_RS03435 613584..614408 - 825 WP_000754758.1 SDR family oxidoreductase -
DLJ64_RS03440 614491..614745 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DLJ64_RS03445 614742..614993 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DLJ64_RS03450 615276..615326 + 51 WP_001364200.1 his operon leader peptide -
DLJ64_RS03460 615472..616371 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
DLJ64_RS03465 616377..617681 + 1305 WP_021523144.1 histidinol dehydrogenase -
DLJ64_RS03470 617678..618748 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
DLJ64_RS03475 618748..619815 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T105873 WP_000767829.1 NZ_CP029579:c614745-614491 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T105873 NZ_CP029579:c614745-614491 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT105873 WP_001259255.1 NZ_CP029579:c614993-614742 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT105873 NZ_CP029579:c614993-614742 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References