Detailed information of TA system TA105860

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54861..55130
• Replicon: plasmid pDA33135-70
• Accession: NZ_CP029578
• Organism: Escherichia coli strain DA33135

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DLJ63_RS27470
• Protein ID: WP_001312861.1
• Coordinates: 54972..55130 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	54861..54926 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ63_RS27435	49879..50328	-	450	WP_110046579.1	hypothetical protein	-
DLJ63_RS27445	50630..51157	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
DLJ63_RS27450	51215..51448	+	234	WP_000006003.1	DUF905 family protein	-
DLJ63_RS27455	51509..53473	+	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
DLJ63_RS27460	53542..53976	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
DLJ63_RS27465	53973..54692	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	54704..54928	+	225	NuclAT_0	-	-
-	54704..54928	+	225	NuclAT_0	-	-
-	54704..54928	+	225	NuclAT_0	-	-
-	54704..54928	+	225	NuclAT_0	-	-
DLJ63_RS27875	54713..54892	-	180	WP_001309233.1	hypothetical protein	-
-	54861..54926	+	66	-	-	Antitoxin
DLJ63_RS27470	54972..55130	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
DLJ63_RS28055	55368..55745	-	378	Protein_69	hypothetical protein	-
DLJ63_RS27490	56045..56341	+	297	WP_001272251.1	hypothetical protein	-
DLJ63_RS27495	56452..57273	+	822	WP_001234445.1	DUF945 domain-containing protein	-
DLJ63_RS27500	57570..58172	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
DLJ63_RS27510	58495..58878	+	384	WP_001354030.1	relaxosome protein TraM	-
DLJ63_RS27515	59072..59743	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
DLJ63_RS27520	59880..60107	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..70197	70197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T105860 WP_001312861.1 NZ_CP029578:54972-55130 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T105860 NZ_CP029578:54972-55130 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT105860 NZ_CP029578:54861-54926 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin