Detailed information of TA system TA105854

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26880..27133
• Replicon: plasmid pDA33135-70
• Accession: NZ_CP029578
• Organism: Escherichia coli strain DA33135

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: DLJ63_RS27245
• Protein ID: WP_001312851.1
• Coordinates: 26984..27133 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26880..26939 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ63_RS27200	22791..23351	+	561	WP_000139321.1	fertility inhibition protein FinO	-
DLJ63_RS27205	23480..23692	+	213	WP_001309245.1	ANR family transcriptional regulator	-
DLJ63_RS27210	23937..24398	+	462	WP_001233838.1	thermonuclease family protein	-
DLJ63_RS27215	24444..24653	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
DLJ63_RS27220	24691..24900	+	210	Protein_26	hypothetical protein	-
DLJ63_RS27225	24964..25668	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
DLJ63_RS27230	25723..26109	+	387	Protein_28	DUF2726 domain-containing protein	-
DLJ63_RS27235	26264..26737	+	474	WP_016240489.1	hypothetical protein	-
-	26880..26939	-	60	NuclAT_1	-	Antitoxin
-	26880..26939	-	60	NuclAT_1	-	Antitoxin
-	26880..26939	-	60	NuclAT_1	-	Antitoxin
-	26880..26939	-	60	NuclAT_1	-	Antitoxin
DLJ63_RS27245	26984..27133	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
DLJ63_RS27250	27418..27666	+	249	WP_000083839.1	replication regulatory protein RepA	-
DLJ63_RS27260	27911..27985	+	75	WP_001365705.1	RepA leader peptide Tap	-
DLJ63_RS27265	27978..28835	+	858	WP_162543890.1	incFII family plasmid replication initiator RepA	-
DLJ63_RS27275	29748..30017	+	270	WP_012881119.1	ABC transporter ATPase	-
DLJ63_RS27280	30014..30295	+	282	WP_012881118.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DLJ63_RS27285	30341..31189	+	849	WP_012881117.1	3'-5' exonuclease	-
DLJ63_RS27290	31306..31788	+	483	WP_001311056.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..70197	70197
	-	flank	IS/Tn	-	-	24964..25668	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T105854 WP_001312851.1 NZ_CP029578:26984-27133 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T105854 NZ_CP029578:26984-27133 [Escherichia coli]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT105854 NZ_CP029578:c26939-26880 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin