Detailed information of TA system TA105823

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 121338..121592
• Replicon: plasmid pDA33133-157
• Accession: NZ_CP029575
• Organism: Escherichia coli strain DA33133

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: DLJ62_RS25320
• Protein ID: WP_015387399.1
• Coordinates: 121386..121592 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	121338..121399 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ62_RS25270	116990..117202	+	213	WP_005012601.1	hypothetical protein	-
DLJ62_RS25995	117503..117592	-	90	Protein_141	IS1 family transposase	-
DLJ62_RS25285	117647..118324	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
DLJ62_RS25290	118324..118671	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
DLJ62_RS25295	118691..120262	+	1572	WP_023149734.1	IS66 family transposase	-
DLJ62_RS25300	120300..120916	-	617	Protein_145	IS1 family transposase	-
DLJ62_RS25310	121017..121199	+	183	WP_000968309.1	hypothetical protein	-
-	121338..121399	-	62	NuclAT_2	-	Antitoxin
-	121338..121399	-	62	NuclAT_2	-	Antitoxin
-	121338..121399	-	62	NuclAT_2	-	Antitoxin
-	121338..121399	-	62	NuclAT_2	-	Antitoxin
DLJ62_RS25320	121386..121592	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
DLJ62_RS25325	121876..122133	+	258	WP_000083833.1	replication regulatory protein RepA	-
DLJ62_RS25335	122369..122443	+	75	WP_032336874.1	RepA leader peptide Tap	-
DLJ62_RS25340	122436..122882	+	447	Protein_150	incFII family plasmid replication initiator RepA	-
DLJ62_RS25345	122828..123310	-	483	Protein_151	VENN motif pre-toxin domain-containing protein	-
DLJ62_RS25350	124203..125423	+	1221	WP_000410951.1	arginine deiminase	-
DLJ62_RS25355	125434..126345	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / blaCTX-M-15 / aac(3)-IId / mph(A) / sul1 / qacE / aadA5 / dfrA17 / aac(6')-Ib-cr / blaOXA-1	-	1..156518	156518

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T105823 WP_015387399.1 NZ_CP029575:121386-121592 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T105823 NZ_CP029575:121386-121592 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT105823 NZ_CP029575:c121399-121338 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin