Detailed information of TA system TA105819

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81284..81517
• Replicon: plasmid pDA33133-157
• Accession: NZ_CP029575
• Organism: Escherichia coli strain DA33133

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DLJ62_RS25065
• Protein ID: WP_001312861.1
• Coordinates: 81359..81517 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	81284..81315 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ62_RS25025	76573..77277	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
DLJ62_RS25035	77405..77902	+	498	WP_042039995.1	single-stranded DNA-binding protein	-
DLJ62_RS25040	77970..78203	+	234	WP_000005990.1	DUF905 family protein	-
DLJ62_RS25045	78231..78428	+	198	Protein_96	hypothetical protein	-
DLJ62_RS25050	78483..78917	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
DLJ62_RS25055	78914..79633	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
DLJ62_RS25845	79645..79833	-	189	WP_001299721.1	hypothetical protein	-
-	79645..79842	+	198	NuclAT_0	-	-
-	79645..79842	+	198	NuclAT_0	-	-
-	79645..79842	+	198	NuclAT_0	-	-
-	79645..79842	+	198	NuclAT_0	-	-
DLJ62_RS25060	79890..81259	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	81284..81315	+	32	NuclAT_1	-	Antitoxin
-	81284..81315	+	32	NuclAT_1	-	Antitoxin
-	81284..81315	+	32	NuclAT_1	-	Antitoxin
-	81284..81315	+	32	NuclAT_1	-	Antitoxin
DLJ62_RS25065	81359..81517	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
DLJ62_RS25080	82438..82725	+	288	WP_000107535.1	hypothetical protein	-
DLJ62_RS25085	82843..83664	+	822	WP_001234426.1	DUF945 domain-containing protein	-
DLJ62_RS25090	83961..84563	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
DLJ62_RS25095	84884..85267	+	384	WP_001151524.1	relaxosome protein TraM	-
DLJ62_RS25100	85454..86143	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / blaCTX-M-15 / aac(3)-IId / mph(A) / sul1 / qacE / aadA5 / dfrA17 / aac(6')-Ib-cr / blaOXA-1	-	1..156518	156518

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T105819 WP_001312861.1 NZ_CP029575:81359-81517 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T105819 NZ_CP029575:81359-81517 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT105819 NZ_CP029575:81284-81315 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin