Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3883629..3884131 Replicon chromosome
Accession NZ_CP029574
Organism Escherichia coli strain DA33133

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag DLJ62_RS20110 Protein ID WP_000767831.1
Coordinates 3883629..3883883 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DLJ62_RS20115 Protein ID WP_001259255.1
Coordinates 3883880..3884131 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ62_RS20080 3878644..3878871 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DLJ62_RS20085 3878885..3879943 - 1059 WP_000492321.1 transport protein YeeE -
DLJ62_RS20090 3880122..3881480 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DLJ62_RS20095 3881470..3881532 - 63 WP_010723108.1 membrane protein YoeI -
DLJ62_RS20100 3881747..3882676 - 930 WP_024195433.1 LysR family transcriptional regulator -
DLJ62_RS20105 3882722..3883546 - 825 WP_000754737.1 SDR family oxidoreductase -
DLJ62_RS20110 3883629..3883883 - 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DLJ62_RS20115 3883880..3884131 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DLJ62_RS20120 3884415..3884465 + 51 WP_001364200.1 his operon leader peptide -
DLJ62_RS20130 3884611..3885510 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DLJ62_RS20135 3885516..3886820 + 1305 WP_001446927.1 histidinol dehydrogenase -
DLJ62_RS20140 3886817..3887887 + 1071 WP_110062746.1 histidinol-phosphate transaminase -
DLJ62_RS20145 3887887..3888954 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T105815 WP_000767831.1 NZ_CP029574:c3883883-3883629 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T105815 NZ_CP029574:c3883883-3883629 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT105815 WP_001259255.1 NZ_CP029574:c3884131-3883880 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT105815 NZ_CP029574:c3884131-3883880 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References