Detailed information of TA system TA105803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1985959..1986653
• Replicon: chromosome
• Accession: NZ_CP029574
• Organism: Escherichia coli strain DA33133

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: DLJ62_RS10155
• Protein ID: WP_001263489.1
• Coordinates: 1986255..1986653 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: DLJ62_RS10150
• Protein ID: WP_000554758.1
• Coordinates: 1985959..1986252 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ62_RS10130	1981591..1982088	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
DLJ62_RS10135	1982312..1984024	-	1713	Protein_1828	flagellar biosynthesis protein FlhA	-
DLJ62_RS10140	1983996..1984781	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
DLJ62_RS10145	1984852..1985907	+	1056	WP_001226164.1	DNA polymerase IV	-
DLJ62_RS10150	1985959..1986252	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DLJ62_RS10155	1986255..1986653	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DLJ62_RS10160	1986663..1987115	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
DLJ62_RS10165	1987433..1987639	+	207	Protein_1834	RtcB family protein	-
DLJ62_RS10170	1987635..1988156	+	522	Protein_1835	peptide chain release factor H	-
DLJ62_RS10175	1988213..1989670	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
DLJ62_RS10180	1989931..1990389	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1990985..1991065	+	81	NuclAT_12	-	-
-	1990985..1991065	+	81	NuclAT_12	-	-
-	1990985..1991065	+	81	NuclAT_12	-	-
-	1990985..1991065	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	1971374..2004941	33567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T105803 WP_001263489.1 NZ_CP029574:1986255-1986653 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T105803 NZ_CP029574:1986255-1986653 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT105803 WP_000554758.1 NZ_CP029574:1985959-1986252 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT105803 NZ_CP029574:1985959-1986252 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3