Detailed information of TA system TA105794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 25790..26448
• Replicon: plasmid pDA33098-71
• Accession: NZ_CP029571
• Organism: Acinetobacter baumannii strain DA33098

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DLD53_RS20330
• Protein ID: WP_000312250.1
• Coordinates: 26089..26448 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DLD53_RS20325
• Protein ID: WP_001096429.1
• Coordinates: 25790..26089 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLD53_RS20285	21537..22562	+	1026	WP_001988464.1	IS30-like element ISAba125 family transposase	-
DLD53_RS20290	22564..23033	+	470	Protein_31	hypothetical protein	-
DLD53_RS20295	23009..23188	+	180	WP_000387630.1	hypothetical protein	-
DLD53_RS20300	23205..23834	+	630	WP_000701003.1	hypothetical protein	-
DLD53_RS20305	23874..24092	+	219	WP_001043201.1	hypothetical protein	-
DLD53_RS20310	24112..24666	+	555	WP_000790084.1	hypothetical protein	-
DLD53_RS20315	24716..25252	+	537	WP_000731978.1	hypothetical protein	-
DLD53_RS20325	25790..26089	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
DLD53_RS20330	26089..26448	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DLD53_RS20335	26649..27215	+	567	WP_000710385.1	hypothetical protein	-
DLD53_RS20340	27264..27446	+	183	WP_000373385.1	hypothetical protein	-
DLD53_RS20345	27513..28211	+	699	WP_000873188.1	hypothetical protein	-
DLD53_RS20350	28350..28733	+	384	WP_000654348.1	hypothetical protein	-
DLD53_RS20355	28800..29558	+	759	WP_001053127.1	hypothetical protein	-
DLD53_RS20365	30613..30927	+	315	WP_000708714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa	-	1..71234	71234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T105794 WP_000312250.1 NZ_CP029571:c26448-26089 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T105794 NZ_CP029571:c26448-26089 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT105794 WP_001096429.1 NZ_CP029571:c26089-25790 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT105794 NZ_CP029571:c26089-25790 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA