Detailed information of TA system TA105759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3492541..3493127
• Replicon: chromosome
• Accession: NZ_CP029550
• Organism: Methylobacterium durans strain 17SD2-17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DK389_RS16105
• Protein ID: WP_109891065.1
• Coordinates: 3492846..3493127 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DK389_RS16100
• Protein ID: WP_109891063.1
• Coordinates: 3492541..3492834 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DK389_RS16085	3490111..3490344	+	234	WP_109891057.1	hypothetical protein	-
DK389_RS16090	3490325..3491557	+	1233	WP_109891059.1	HNH endonuclease	-
DK389_RS16095	3491564..3492037	+	474	WP_162560679.1	hypothetical protein	-
DK389_RS16100	3492541..3492834	-	294	WP_109891063.1	HigA family addiction module antidote protein	Antitoxin
DK389_RS16105	3492846..3493127	-	282	WP_109891065.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DK389_RS16110	3493919..3494479	-	561	Protein_3214	hypothetical protein	-
DK389_RS16115	3494529..3494915	-	387	WP_109891067.1	hypothetical protein	-
DK389_RS32475	3495630..3496304	-	675	WP_162560680.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kdsA	3418293..3503765	85472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (20-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-82)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10713.12 Da
• Isoelectric Point: 9.3531

>T105759 WP_109891065.1 NZ_CP029550:c3493127-3492846 [Methylobacterium durans]
MIASFRNAAARSAWKRRFIEGVPNDIMKVTFRKLTQLHNARSLDDLRAPPGNRLEALSGDRAGQHSLRVNDQWRLCFRCQ
DGDAFDVELVDYH

Nucleotide

• Length: 282 bp

>T105759 NZ_CP029550:c3493127-3492846 [Methylobacterium durans]
ATGATCGCGAGCTTCCGGAACGCTGCGGCCCGTTCCGCCTGGAAGCGTCGCTTCATCGAAGGTGTCCCGAACGACATCAT
GAAAGTGACGTTCCGCAAGCTGACGCAGCTTCATAACGCCCGAAGCTTGGATGACCTTCGGGCGCCGCCGGGCAATCGCC
TCGAGGCCCTGTCTGGAGATCGAGCCGGTCAACACAGCTTGCGCGTCAACGATCAGTGGCGGCTGTGTTTCCGATGTCAG
GATGGCGACGCCTTCGATGTTGAGCTGGTTGATTATCATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10779.41 Da
• Isoelectric Point: 5.8315

>AT105759 WP_109891063.1 NZ_CP029550:c3492834-3492541 [Methylobacterium durans]
MADFAPTHPGEVLREDFLLPLGLSQYALAKAIAVPETRISEIIRGRRSVSAETALRLARYFGTSPEFWMGMQATYDLEMA
RDATAPLIEKQVQPRAA

Nucleotide

• Length: 294 bp

>AT105759 NZ_CP029550:c3492834-3492541 [Methylobacterium durans]
ATGGCAGATTTTGCTCCGACACATCCTGGCGAGGTACTGCGAGAGGATTTTCTCCTGCCGCTGGGCCTCAGCCAGTATGC
TTTGGCCAAAGCTATCGCCGTGCCAGAGACTCGCATCAGCGAGATCATTCGAGGCAGGCGTTCGGTGTCGGCTGAGACGG
CTCTGCGGCTCGCCCGCTATTTCGGCACGTCCCCGGAATTCTGGATGGGCATGCAGGCGACGTATGATCTCGAGATGGCG
CGGGATGCTACCGCTCCGTTGATCGAGAAGCAGGTTCAACCGCGAGCCGCGTGA