Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 2309427..2309991 Replicon chromosome
Accession NZ_CP029536
Organism Lacticaseibacillus paracasei strain LC355

Toxin (Protein)


Gene name yafQ Uniprot ID A0A510WIJ9
Locus tag DKM32_RS11985 Protein ID WP_016363448.1
Coordinates 2309689..2309991 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag DKM32_RS11980 Protein ID WP_003570051.1
Coordinates 2309427..2309696 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKM32_RS11950 2304432..2305319 - 888 WP_003570041.1 ABC transporter ATP-binding protein -
DKM32_RS11955 2305516..2306742 - 1227 WP_003570043.1 hypothetical protein -
DKM32_RS11960 2306940..2307344 + 405 WP_003570045.1 MerR family transcriptional regulator -
DKM32_RS11965 2307337..2307663 + 327 WP_003584272.1 YrdB family protein -
DKM32_RS11970 2307726..2308355 + 630 WP_003570047.1 DUF2399 domain-containing protein -
DKM32_RS11975 2308400..2309053 + 654 WP_003570049.1 GNAT family N-acetyltransferase -
DKM32_RS11980 2309427..2309696 + 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
DKM32_RS11985 2309689..2309991 + 303 WP_016363448.1 type II toxin-antitoxin system YafQ family toxin Toxin
DKM32_RS16275 2310330..2310473 + 144 WP_162259784.1 hypothetical protein -
DKM32_RS11990 2310591..2311283 + 693 WP_003570055.1 hypothetical protein -
DKM32_RS11995 2311519..2313696 + 2178 WP_003570057.1 RNA-binding transcriptional accessory protein -
DKM32_RS12000 2314121..2314678 - 558 WP_003584278.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-97)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11510.33 Da        Isoelectric Point: 9.8881

>T105755 WP_016363448.1 NZ_CP029536:2309689-2309991 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELYVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLVK

Download         Length: 303 bp

>T105755 NZ_CP029536:2309689-2309991 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCCGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCCT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTATATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTGAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGTTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT105755 WP_003570051.1 NZ_CP029536:2309427-2309696 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT105755 NZ_CP029536:2309427-2309696 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAGGAAACTCGCTTGAATATTCGTGTTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A510WIJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References