Detailed information of TA system TA105754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 1648160..1648676
• Replicon: chromosome
• Accession: NZ_CP029536
• Organism: Lacticaseibacillus paracasei strain LC355

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A243Q0H7
• Locus tag: DKM32_RS08445
• Protein ID: WP_003573774.1
• Coordinates: 1648395..1648676 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: DKM32_RS08440
• Protein ID: WP_016363783.1
• Coordinates: 1648160..1648402 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DKM32_RS08420	1643614..1645785	-	2172	WP_003607130.1	class 1b ribonucleoside-diphosphate reductase subunit alpha	-
DKM32_RS08425	1645957..1646946	+	990	WP_003586769.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
DKM32_RS08430	1646943..1647428	+	486	WP_003586772.1	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	-
DKM32_RS08440	1648160..1648402	+	243	WP_016363783.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DKM32_RS08445	1648395..1648676	+	282	WP_003573774.1	type II toxin-antitoxin system YafQ family toxin	Toxin
DKM32_RS08455	1649058..1649309	+	252	WP_003586983.1	IS3 family transposase	-
DKM32_RS08460	1649363..1650205	+	843	WP_029327644.1	IS3 family transposase	-
DKM32_RS08465	1650326..1650628	+	303	Protein_1540	DUF1722 domain-containing protein	-
DKM32_RS08470	1650873..1651982	-	1110	WP_003607129.1	hypothetical protein	-
DKM32_RS08475	1651984..1653426	-	1443	WP_003586782.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1544693..1681948	137255
	-	flank	IS/Tn	-	-	1642033..1643448	1415
	-	flank	IS/Tn	-	-	1649058..1649309	251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11371.98 Da
• Isoelectric Point: 9.5081

>T105754 WP_003573774.1 NZ_CP029536:1648395-1648676 [Lacticaseibacillus paracasei]
MNKFVYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T105754 NZ_CP029536:1648395-1648676 [Lacticaseibacillus paracasei]
ATGAATAAATTTGTTTATAAACCAGCCTTTGAACGTCAATTTAAAAAACATTACAAGACGATTTTAAAAGGGGGGCGCTA
TAAAAAAGAAGATTTTGAAGCTGTTTATCATAAACTATTACGAGATGAACCGTTAGATCCGCATTATAATGATCACGCTT
TGGTTAATCGTAAACCAGAGCGTGATTTACATATCAAGCCGGATTGGTTATTAATTTACAAATATGATGGTGAGTTTGTG
CGCTTTATCGACACGGGTTCGCATTCAGATCTTTTTAACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9217.44 Da
• Isoelectric Point: 5.1548

>AT105754 WP_016363783.1 NZ_CP029536:1648160-1648402 [Lacticaseibacillus paracasei]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT105754 NZ_CP029536:1648160-1648402 [Lacticaseibacillus paracasei]
ATGACTAGCCAAGTACATTTCAGAATGAGCGAAAAAGAAAAAAAGGACTTCGAGATTGTCCTAAGTCGTGTGGGGTTAAC
TCCCGGGGAAGCTTTTCGTATCTTTGCCAAGAAATCCATTGAAGCCGGAGGAATTCCCTTTGAGGTCTCTCAGCCAAACA
CACGTTTAAAAAAGGCTATTAGGAGTCAAGATTACGTTGAATTTAACGATCCTGAGGAAGGACTGGATTGGTTAAATGAA
TAA

Structures

Toxin

• AlphaFold DB: A0A243Q0H7