Detailed information of TA system TA105747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3486147..3486841
• Replicon: chromosome
• Accession: NZ_CP029492
• Organism: Escherichia coli strain HS30-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: DKQ63_RS18200
• Protein ID: WP_001263493.1
• Coordinates: 3486147..3486545 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: DKQ63_RS18205
• Protein ID: WP_000554757.1
• Coordinates: 3486548..3486841 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DKQ63_RS18170	3481147..3482391	-	1245	WP_000189541.1	esterase FrsA	-
-	3481807..3481887	-	81	NuclAT_8	-	-
-	3481807..3481887	-	81	NuclAT_8	-	-
-	3481807..3481887	-	81	NuclAT_8	-	-
-	3481807..3481887	-	81	NuclAT_8	-	-
DKQ63_RS18175	3482483..3482941	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
DKQ63_RS18180	3483202..3484659	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
DKQ63_RS18185	3484716..3485237	-	522	Protein_3308	peptide chain release factor H	-
DKQ63_RS18190	3485236..3485439	-	204	Protein_3309	RNA ligase RtcB family protein	-
DKQ63_RS18195	3485685..3486137	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
DKQ63_RS18200	3486147..3486545	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DKQ63_RS18205	3486548..3486841	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DKQ63_RS18210	3486893..3487948	-	1056	WP_001226164.1	DNA polymerase IV	-
DKQ63_RS18215	3488019..3488804	-	786	Protein_3314	putative lateral flagellar export/assembly protein LafU	-
DKQ63_RS18220	3488776..3490488	+	1713	Protein_3315	flagellar biosynthesis protein FlhA	-
DKQ63_RS18225	3490712..3491209	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T105747 WP_001263493.1 NZ_CP029492:c3486545-3486147 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T105747 NZ_CP029492:c3486545-3486147 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT105747 WP_000554757.1 NZ_CP029492:c3486841-3486548 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT105747 NZ_CP029492:c3486841-3486548 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6