Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1725129..1725631 Replicon chromosome
Accession NZ_CP029492
Organism Escherichia coli strain HS30-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DKQ63_RS08750 Protein ID WP_000767829.1
Coordinates 1725377..1725631 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DKQ63_RS08745 Protein ID WP_001259255.1
Coordinates 1725129..1725380 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKQ63_RS08715 1720307..1721374 - 1068 WP_001610379.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
DKQ63_RS08720 1721374..1722444 - 1071 WP_001614841.1 histidinol-phosphate transaminase -
DKQ63_RS08725 1722441..1723745 - 1305 WP_001515511.1 histidinol dehydrogenase -
DKQ63_RS08730 1723751..1724650 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
DKQ63_RS08740 1724796..1724846 - 51 WP_001364200.1 his operon leader peptide -
DKQ63_RS08745 1725129..1725380 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DKQ63_RS08750 1725377..1725631 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DKQ63_RS08755 1725714..1726538 + 825 WP_000754749.1 SDR family oxidoreductase -
DKQ63_RS08760 1726584..1727513 + 930 WP_000803351.1 LysR family transcriptional regulator -
DKQ63_RS08765 1727728..1727790 + 63 WP_010723108.1 membrane protein YoeI -
DKQ63_RS08770 1727780..1729138 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DKQ63_RS08775 1729317..1730375 + 1059 WP_110991435.1 transport protein YeeE -
DKQ63_RS08780 1730389..1730616 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T105735 WP_000767829.1 NZ_CP029492:1725377-1725631 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T105735 NZ_CP029492:1725377-1725631 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT105735 WP_001259255.1 NZ_CP029492:1725129-1725380 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT105735 NZ_CP029492:1725129-1725380 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References