Detailed information of TA system TA105710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 519750..520354
• Replicon: chromosome
• Accession: NZ_CP029482
• Organism: Pseudomonas sp. 31-12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DJ564_RS02415
• Protein ID: WP_109635921.1
• Coordinates: 519750..520064 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DJ564_RS02420
• Protein ID: WP_109627422.1
• Coordinates: 520064..520354 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DJ564_RS02385	514783..516069	+	1287	WP_109627416.1	OprD family porin	-
DJ564_RS02390	516301..517050	+	750	WP_094469618.1	2OG-Fe dioxygenase family protein	-
DJ564_RS02395	517097..517510	+	414	WP_178082335.1	GNAT family N-acetyltransferase	-
DJ564_RS02400	517507..518223	+	717	WP_109627418.1	AzlC family ABC transporter permease	-
DJ564_RS02405	518202..518519	+	318	WP_094469616.1	AzlD domain-containing protein	-
DJ564_RS02410	518436..519362	-	927	WP_109627420.1	LysR family transcriptional regulator	-
DJ564_RS02415	519750..520064	+	315	WP_109635921.1	hypothetical protein	Toxin
DJ564_RS02420	520064..520354	+	291	WP_109627422.1	helix-turn-helix domain-containing protein	Antitoxin
DJ564_RS31920	520389..520544	+	156	WP_162556160.1	hypothetical protein	-
DJ564_RS02425	520883..521896	+	1014	WP_109627424.1	2-keto-3-deoxygluconate permease	-
DJ564_RS02430	521893..523173	+	1281	WP_109627426.1	four-carbon acid sugar kinase family protein	-
DJ564_RS02435	523179..524180	+	1002	WP_109635923.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
DJ564_RS02440	524236..525009	+	774	WP_109627427.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-72)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11620.45 Da
• Isoelectric Point: 9.3950

>T105710 WP_109635921.1 NZ_CP029482:519750-520064 [Pseudomonas sp. 31-12]
MIFIETLIFTADLPSHLGDQEYSEFQQYLAEHPEAGALIEGTGGLRKIRWAAHGKGKSAGVRVIYYHVTSAFQIRLLLIY
RKGIQDSLSGKEKAVLCAINKGWK

Nucleotide

• Length: 315 bp

>T105710 NZ_CP029482:519750-520064 [Pseudomonas sp. 31-12]
ATGATCTTCATCGAGACGTTAATTTTTACTGCTGACCTACCCAGCCATCTTGGTGACCAAGAGTACAGTGAGTTTCAGCA
ATATTTGGCCGAACATCCTGAAGCTGGAGCTTTGATTGAAGGAACCGGTGGGCTGAGAAAAATCCGCTGGGCAGCGCATG
GCAAAGGTAAGAGTGCCGGTGTGCGAGTCATCTACTATCACGTAACCTCGGCTTTTCAGATACGTTTGCTACTGATTTAC
CGAAAAGGCATTCAAGACTCCCTTTCGGGTAAAGAAAAAGCAGTTCTTTGTGCCATCAATAAAGGATGGAAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10965.45 Da
• Isoelectric Point: 7.3208

>AT105710 WP_109627422.1 NZ_CP029482:520064-520354 [Pseudomonas sp. 31-12]
MEKNLFDRLVESMTQMDEIDRGERQPSREFHVDAVQVKKIRLATGLSQAAFAKRIDVAVGTLRNWEQGRRDPEGPARALL
RAIYNDPEHVLAALNH

Nucleotide

• Length: 291 bp

>AT105710 NZ_CP029482:520064-520354 [Pseudomonas sp. 31-12]
ATGGAAAAAAATCTCTTCGATCGTCTCGTTGAAAGCATGACGCAGATGGATGAGATCGACCGTGGTGAGCGGCAGCCATC
GCGTGAATTCCATGTCGATGCAGTTCAGGTCAAAAAAATTCGCCTCGCCACGGGACTGTCCCAGGCCGCTTTTGCAAAGA
GAATTGATGTAGCCGTGGGAACGCTTCGGAATTGGGAGCAGGGGCGGCGTGATCCGGAAGGACCTGCCCGTGCTTTGTTA
CGAGCGATTTATAACGATCCTGAGCACGTTTTAGCAGCGCTCAATCACTAA