Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 335632..336149 Replicon chromosome
Accession NZ_CP029474
Organism Staphylococcus aureus strain USA 100 isolate 30-47

Toxin (Protein)


Gene name yoeB Uniprot ID A0A3A3ATA4
Locus tag DKK89_RS01835 Protein ID WP_000113265.1
Coordinates 335632..335898 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID T1YB60
Locus tag DKK89_RS01840 Protein ID WP_000584499.1
Coordinates 335898..336149 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKK89_RS01805 331060..331596 - 537 WP_000169317.1 GNAT family N-acetyltransferase -
DKK89_RS01810 331830..332654 - 825 WP_000572038.1 formate/nitrite transporter family protein -
DKK89_RS01815 332871..333053 - 183 WP_000230292.1 hypothetical protein -
DKK89_RS01820 333137..333604 - 468 WP_000153347.1 SRPBCC domain-containing protein -
DKK89_RS01825 333790..335337 - 1548 WP_000727772.1 zinc ABC transporter substrate-binding lipoprotein AdcA -
DKK89_RS01830 335526..335597 - 72 Protein_338 hypothetical protein -
DKK89_RS01835 335632..335898 - 267 WP_000113265.1 Txe/YoeB family addiction module toxin Toxin
DKK89_RS01840 335898..336149 - 252 WP_000584499.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DKK89_RS01845 336314..336415 - 102 Protein_341 hypothetical protein -
DKK89_RS01850 336422..337021 - 600 WP_000162810.1 DsbA family protein -
DKK89_RS01855 337040..337402 - 363 WP_000819843.1 DUF4467 domain-containing protein -
DKK89_RS01860 337657..338907 + 1251 WP_001012225.1 FemA/FemB family glycyltransferase FmhA -
DKK89_RS01865 339004..339735 - 732 WP_000615461.1 amino acid ABC transporter ATP-binding protein -
DKK89_RS01870 339732..340451 - 720 WP_000479576.1 amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-81)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10436.96 Da        Isoelectric Point: 10.0573

>T105697 WP_000113265.1 NZ_CP029474:c335898-335632 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Download         Length: 267 bp

>T105697 NZ_CP029474:c335898-335632 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9433.60 Da        Isoelectric Point: 4.3407

>AT105697 WP_000584499.1 NZ_CP029474:c336149-335898 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Download         Length: 252 bp

>AT105697 NZ_CP029474:c336149-335898 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BWF

Antitoxin

Source ID Structure
PDB 6L8G
PDB 6L8F
PDB 7BWF
PDB 6L8E
AlphaFold DB T1YB60

References