Detailed information of TA system TA105678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 230959..231570
• Replicon: plasmid pSF25509b
• Accession: NZ_CP029452
• Organism: Sinorhizobium fredii CCBAU 25509

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: AOX55_RS21620
• Protein ID: WP_080577501.1
• Coordinates: 231289..231570 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: AOX55_RS21615
• Protein ID: WP_037395516.1
• Coordinates: 230959..231258 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOX55_RS21585	227801..228664	-	864	WP_037395524.1	Ku protein	-
AOX55_RS21590	228922..229230	+	309	WP_037395522.1	DUF2934 domain-containing protein	-
AOX55_RS21595	229296..229490	+	195	WP_037395520.1	cold-shock protein	-
AOX55_RS21605	229804..230253	-	450	WP_037395518.1	helix-turn-helix transcriptional regulator	-
AOX55_RS21615	230959..231258	-	300	WP_037395516.1	HigA family addiction module antidote protein	Antitoxin
AOX55_RS21620	231289..231570	-	282	WP_080577501.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOX55_RS21625	231613..231813	+	201	WP_037395513.1	tautomerase family protein	-
AOX55_RS21630	232229..232825	-	597	WP_037395511.1	mobile mystery protein B	-
AOX55_RS21635	232822..233283	-	462	WP_037395509.1	mobile mystery protein A	-
AOX55_RS21650	234777..234989	+	213	Protein_230	hypothetical protein	-
AOX55_RS21655	235135..235573	-	439	Protein_231	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / katA	1..2210745	2210745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-80)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10338.70 Da
• Isoelectric Point: 9.8806

>T105678 WP_080577501.1 NZ_CP029452:c231570-231289 [Sinorhizobium fredii CCBAU 25509]
VIRSFKDAKTEAVARGKAPKGIPSDIVRSAVRKLTILNSAAARDDLRSPPGNRLEALKGDREGQHSNRINDQWRICFVWT
AAGIEDVEIVDYH

Nucleotide

• Length: 282 bp

>T105678 NZ_CP029452:c231570-231289 [Sinorhizobium fredii CCBAU 25509]
GTGATCAGGTCATTTAAAGACGCCAAAACTGAAGCTGTTGCACGCGGCAAGGCCCCAAAAGGCATTCCTTCAGACATCGT
GAGGTCGGCCGTTCGTAAATTGACGATCCTGAACAGCGCAGCCGCTCGCGACGACCTGCGGTCCCCTCCGGGAAATCGAC
TTGAAGCCCTTAAGGGCGATAGAGAAGGCCAACATTCGAACCGGATCAATGATCAGTGGCGCATATGCTTCGTCTGGACA
GCTGCCGGTATCGAAGACGTCGAAATAGTTGACTATCATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11326.20 Da
• Isoelectric Point: 6.7273

>AT105678 WP_037395516.1 NZ_CP029452:c231258-230959 [Sinorhizobium fredii CCBAU 25509]
MTHILPPVHPGEILREEYLLPLNMSAGSLAKKLNVPRTRIERIAAEQTPITTDTALRLAKYFRTTPEFWMKMQTSFNLKT
EEIALHGELELIPEIDRAA

Nucleotide

• Length: 300 bp

>AT105678 NZ_CP029452:c231258-230959 [Sinorhizobium fredii CCBAU 25509]
ATGACCCATATCCTTCCGCCGGTCCATCCGGGCGAAATCCTCCGCGAGGAGTACCTACTTCCTCTGAACATGAGCGCCGG
TTCACTGGCGAAAAAGCTCAATGTTCCTCGCACCCGCATCGAACGTATAGCGGCGGAGCAAACACCGATAACGACGGATA
CCGCTCTGCGCCTCGCCAAGTATTTCCGCACAACGCCGGAATTTTGGATGAAGATGCAGACCAGCTTCAACCTCAAGACA
GAGGAAATAGCGCTTCATGGCGAACTGGAGTTGATCCCGGAAATCGATAGAGCAGCATAG