Detailed information of TA system TA105639

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 84732..84971
• Replicon: plasmid unnamed2
• Accession: NZ_CP029422
• Organism: Escherichia coli strain 3385

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: DFS94_RS25445
• Protein ID: WP_023144756.1
• Coordinates: 84837..84971 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	84732..84792 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DFS94_RS25410	80523..81083	+	561	WP_000139341.1	fertility inhibition protein FinO	-
DFS94_RS25415	81214..81426	+	213	WP_013023861.1	hypothetical protein	-
DFS94_RS25425	81985..82410	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
DFS94_RS25430	82407..82757	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
DFS94_RS25435	82788..84401	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
DFS94_RS25440	84479..84765	+	287	Protein_93	DUF2726 domain-containing protein	-
-	84732..84792	-	61	NuclAT_2	-	Antitoxin
-	84732..84792	-	61	NuclAT_2	-	Antitoxin
-	84732..84792	-	61	NuclAT_2	-	Antitoxin
-	84732..84792	-	61	NuclAT_2	-	Antitoxin
DFS94_RS25445	84837..84971	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
DFS94_RS25450	85268..85522	+	255	WP_000083850.1	replication regulatory protein RepA	-
DFS94_RS25835	85628..85762	+	135	Protein_96	protein CopA/IncA	-
DFS94_RS25455	85759..85833	+	75	WP_031943482.1	RepA leader peptide Tap	-
DFS94_RS25460	85826..86683	+	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
DFS94_RS25470	87623..88276	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
DFS94_RS25475	88369..88626	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
DFS94_RS25480	88559..88960	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..89323	89323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T105639 WP_023144756.1 NZ_CP029422:84837-84971 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T105639 NZ_CP029422:84837-84971 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT105639 NZ_CP029422:c84792-84732 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin