Detailed information of TA system TA105635

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57533..57775
• Replicon: plasmid unnamed2
• Accession: NZ_CP029422
• Organism: Escherichia coli strain 3385

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DFS94_RS25285
• Protein ID: WP_001312861.1
• Coordinates: 57617..57775 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	57533..57573 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DFS94_RS25265	52728..54686	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
DFS94_RS25270	54741..55175	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
DFS94_RS25275	55172..55891	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	55903..56089	+	187	NuclAT_0	-	-
-	55903..56089	+	187	NuclAT_0	-	-
-	55903..56089	+	187	NuclAT_0	-	-
-	55903..56089	+	187	NuclAT_0	-	-
DFS94_RS25280	56137..57506	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	57533..57573	+	41	NuclAT_1	-	Antitoxin
-	57533..57573	+	41	NuclAT_1	-	Antitoxin
-	57533..57573	+	41	NuclAT_1	-	Antitoxin
-	57533..57573	+	41	NuclAT_1	-	Antitoxin
DFS94_RS25285	57617..57775	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
DFS94_RS25300	58466..58672	+	207	WP_000275859.1	hypothetical protein	-
DFS94_RS25305	58697..58984	+	288	WP_000107535.1	hypothetical protein	-
DFS94_RS25310	59103..59924	+	822	WP_001234469.1	DUF945 domain-containing protein	-
DFS94_RS25315	60221..60823	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
DFS94_RS25320	61154..61537	+	384	WP_001151566.1	relaxosome protein TraM	-
DFS94_RS25325	61671..62348	+	678	WP_001348626.1	PAS domain-containing protein	-
DFS94_RS25330	62436..62663	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..89323	89323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T105635 WP_001312861.1 NZ_CP029422:57617-57775 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T105635 NZ_CP029422:57617-57775 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT105635 NZ_CP029422:57533-57573 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin