Detailed information of TA system TA105623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3243725..3244327
• Replicon: chromosome
• Accession: NZ_CP029420
• Organism: Escherichia coli strain 3385

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: DFS94_RS16160
• Protein ID: WP_000897302.1
• Coordinates: 3243725..3244036 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DFS94_RS16165
• Protein ID: WP_000356397.1
• Coordinates: 3244037..3244327 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DFS94_RS16135	3239639..3240238	+	600	WP_001296610.1	glucose-1-phosphatase	-
DFS94_RS16140	3240232..3241104	+	873	WP_000920762.1	virulence factor BrkB family protein	-
DFS94_RS16145	3241101..3241538	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DFS94_RS16150	3241583..3242524	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DFS94_RS16155	3242588..3243496	-	909	WP_001385591.1	alpha/beta hydrolase	-
DFS94_RS16160	3243725..3244036	+	312	WP_000897302.1	hypothetical protein	Toxin
DFS94_RS16165	3244037..3244327	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
DFS94_RS16175	3244686..3244964	+	279	WP_001296612.1	hypothetical protein	-
DFS94_RS16185	3245361..3245579	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
DFS94_RS16190	3245764..3246213	-	450	WP_032140890.1	hypothetical protein	-
DFS94_RS16195	3246529..3247377	-	849	WP_001038650.1	hypothetical protein	-
DFS94_RS16200	3247667..3247909	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
DFS94_RS16210	3248091..3249020	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T105623 WP_000897302.1 NZ_CP029420:3243725-3244036 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T105623 NZ_CP029420:3243725-3244036 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT105623 WP_000356397.1 NZ_CP029420:3244037-3244327 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT105623 NZ_CP029420:3244037-3244327 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416