Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2392014..2392741 Replicon chromosome
Accession NZ_CP029420
Organism Escherichia coli strain 3385

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag DFS94_RS11965 Protein ID WP_000550189.1
Coordinates 2392427..2392741 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag DFS94_RS11960 Protein ID WP_138976290.1
Coordinates 2392014..2392430 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DFS94_RS11945 2389174..2389839 + 666 Protein_2304 NADPH-dependent 2,4-dienoyl-CoA reductase -
DFS94_RS11950 2389810..2390507 - 698 Protein_2305 IS1 family transposase -
DFS94_RS11955 2390563..2391969 + 1407 Protein_2306 NADPH-dependent 2,4-dienoyl-CoA reductase -
DFS94_RS11960 2392014..2392430 - 417 WP_138976290.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
DFS94_RS11965 2392427..2392741 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
DFS94_RS11970 2393026..2394162 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
DFS94_RS11975 2394247..2394750 + 504 WP_001295542.1 M48 family metallopeptidase -
DFS94_RS11980 2394827..2395519 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
DFS94_RS11985 2395598..2396584 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T105620 WP_000550189.1 NZ_CP029420:c2392741-2392427 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T105620 NZ_CP029420:c2392741-2392427 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15057.49 Da        Isoelectric Point: 4.4547

>AT105620 WP_138976290.1 NZ_CP029420:c2392430-2392014 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVFSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT105620 NZ_CP029420:c2392430-2392014 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTTAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References