Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3093980..3094626 Replicon chromosome
Accession NZ_CP029388
Organism Klebsiella pneumoniae subsp. pneumoniae strain SCKP040074

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag CVG30_RS17455 Protein ID WP_004174016.1
Coordinates 3094279..3094626 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A231WWU8
Locus tag CVG30_RS17450 Protein ID WP_004174017.1
Coordinates 3093980..3094279 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CVG30_RS17430 3090186..3090944 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
CVG30_RS17435 3090994..3091824 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
CVG30_RS17440 3091875..3092204 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
CVG30_RS17445 3092409..3093917 + 1509 WP_004174018.1 glycerol-3-phosphate dehydrogenase -
CVG30_RS17450 3093980..3094279 - 300 WP_004174017.1 XRE family transcriptional regulator Antitoxin
CVG30_RS17455 3094279..3094626 - 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CVG30_RS17460 3094802..3097249 - 2448 WP_002920561.1 glycogen phosphorylase -
CVG30_RS17465 3097267..3098700 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T105587 WP_004174016.1 NZ_CP029388:c3094626-3094279 [Klebsiella pneumoniae subsp. pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T105587 NZ_CP029388:c3094626-3094279 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10844.64 Da        Isoelectric Point: 10.1464

>AT105587 WP_004174017.1 NZ_CP029388:c3094279-3093980 [Klebsiella pneumoniae subsp. pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT105587 NZ_CP029388:c3094279-3093980 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A231WWU8

References